Flow Cytometry Basics for the Non-Expert
Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling i...
| Main Authors: | , , |
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| Format: | eBook |
| Language: | English |
| Published: |
Cham
Springer International Publishing
2018, 2018
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| Edition: | 1st ed. 2018 |
| Series: | Techniques in Life Science and Biomedicine for the Non-Expert
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- 1. Flow Cytometry: Definition, History, and Uses in Biotechnology
- 2. Physics of a Flow Cytometer
- 3. The Language of Flow Cytometry and Experimental Setup
- 4. Fluorochrome Choices for Flow Cytometry
- 5. Compensation
- 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry
- 7. Experimental Considerations with Data Sets as Examples
- 8. Cell Enrichment
- 9. Surface and Intracellular Staining Protocols for Flow Cytometry
- 10. Troubleshooting