Microbial Metabolomics Methods and Protocols

This detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass...

Full description

Bibliographic Details
Other Authors: Baidoo, Edward E.K. (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2019, 2019
Edition:1st ed. 2019
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Microbial Metabolomics: A General Overview
  • Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications
  • Metabolomics: A Microbial Physiology and Metabolism Perspective
  • Untargeted Soil Metabolomics Using Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry
  • Fatty Acid Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis
  • Total Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis
  • High-Throughput Solid Phase Microextraction-Liquid Chromatography-Mass Spectrometry for Microbial Untargeted Metabolomics
  • Targeted Metabolomics of Xylose Fermenting Yeasts Based on Mass Spectrometry
  • Exploiting High-Resolution Mass Spectrometry for Targeted Metabolite Quantification and 13C-labeling Metabolism Analysis
  • Quantitative Profiling of Endogenous Metabolites Using Hydrophilic Inter-Action Liquid Chromatography-Tandem Mass Spectrometry (HILIC-MS/MS)
  • Liquid Chromatography and Mass Spectrometry Analysis of Isoprenoid Intermediates in Escherichia coli
  • Determining the Mode of Action of Antimalarial Drugs Using Time-Resolved LC-MS-Based Metabolite Profiling
  • Use of Liquid Chromatography-Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis
  • Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry
  • A High-Throughput Targeted Metabolomics Workflow for the Detection of 200 Polar Metabolites in Central Carbon Metabolism
  • Cluster Analysis of Untargeted Metabolomic Experiments
  • Machine Learning in Untargeted Metabolomics Experiments
  • Dynamic 13C Labeling of Fast Turnover Metabolites for Analysis of Metabolic Fluxes and Metabolite Channeling
  • Genome-Scale 13C Fluxomics Modeling for Metabolic Engineering of Saccharomyces cerevisiae