Protein Gel Detection and Imaging Methods and Protocols
This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyac...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2018, 2018
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| Edition: | 1st ed. 2018 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Detection of Proteins in Polyacrylamide Gels via Pre-Labeling by Isatoic Anhydride
- Fluorescent Protein Visualization Immediately after Gel Electrophoresis using an In-Gel Trichloroethanol Photoreaction with Tryptophan
- Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel
- Zymographic Determination of Intrinsic Specific Activity of Oxidases in Presence of Interfering Proteins
- A Simple Method for Detecting Phosphorylation of Proteins by using ZN2+ -Phos-tag SDS-PAGE at Neutral pH
- Principle and Method of Silver Staining of Proteins Separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
- Heat/Pressure Treatment with Detergents Significantly Increases Curcumin Solubility and Stability: Its Use as an Environment-Friendly Protein Gel Stain
- Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels
- Microwave Assisted Protein Staining, De-Staining and In-Gel/In-Solution Digestion of Proteins
- Fluorescent Staining of Gels
- A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta during Western Blot Analysis
- TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE
- Detection of Glycoproteins in Polyacrylamide Gels using Pro-Q Emerald 300 Dye, A Fluorescent Periodate Schiff-Base Stain
- Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain
- Detection of Multiple Enzymes in Fermentation Broth using Single PAGE Analysis
- Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis
- A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins
- Tem Minute stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct red 81 and Amido Black
- In-Gel Protein Phosphatase Assay using Fluorogenic Substrates
- Protein Stains and Applications
- The Roles of Acetic Acid and Methanol during Fixing and Staining Proteins in a SDS Polyacrylamide Electrophoresis Gel
- Multicolored Prestained Standard Protein Marker Generation using a Variety of Remazol Dyes for Easy Visualization of Protein Bands during SDS-PAGE
- Coomassie-Brilliant Blue Staining of Polyacrylamide Gels
- A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate Polyacrylamide Gel using Coomassie Blue
- Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue
- Silver Staining Techniques of Polyacrylamide Gels
- Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry
- Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis using 8-Quinolinol Stain
- Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate Polyacrylamide Protein Gels using a Household Detergent
- Paper Adsorbents Remove Coomassie Blue from Gel Destain and used Gel Stain in an Environment-Friendly Manner
- Gel Drying Methods
- Stained Gels can be Stored for Several Months in Non-Sealed Polyethylene Bags
- Radiolabeling and Analysis of Labeled Proteins