Autophagy in Differentiation and Tissue Maintenance Methods and Protocols

This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better und...

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Bibliographic Details
Other Authors: Turksen, Kursad (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2019, 2019
Edition:1st ed. 2019
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Visualization and Measurement of Multiple Components of the Autophagy Flux
  • Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells
  • Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells
  • Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement
  • Autophagy in Adipocyte Differentiation
  • Assessing Autophagy in the Leydig Cells
  • Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells
  • Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells
  • Autophagy in Zebrafish Extraocular Muscle Regeneration
  • The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry
  • Cloning of Autophagy-Related MicroRNAs
  • Assays to Monitor Aggrephagy in Drosophila Brain
  • Methods for Monitoring Autophagy in Silkworm Organs
  • Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia
  • Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening
  • Porcine Cell-Free System to Study Mammalian Sperm Mitophagy
  • Monitoring and Measuring Mammalian Autophagy