Olfactory Receptors Methods and Protocols

This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to...

Full description

Bibliographic Details
Other Authors: Simoes de Souza, Fabio Marques (Editor), Antunes, Gabriela (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2018, 2018
Edition:1st ed. 2018
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
LEADER 03625nmm a2200289 u 4500
001 EB001840659
003 EBX01000000000000001004648
005 00000000000000.0
007 cr|||||||||||||||||||||
008 180702 ||| eng
020 |a 9781493986095 
100 1 |a Simoes de Souza, Fabio Marques  |e [editor] 
245 0 0 |a Olfactory Receptors  |h Elektronische Ressource  |b Methods and Protocols  |c edited by Fabio Marques Simoes de Souza, Gabriela Antunes 
250 |a 1st ed. 2018 
260 |a New York, NY  |b Humana  |c 2018, 2018 
300 |a XI, 390 p. 66 illus., 47 illus. in color  |b online resource 
505 0 |a CD36 Neuronal Identity in the Olfactory Epithelium -- Deorphanization of Olfactory Trace Amine-Associated Receptors -- G Protein-Coupled Receptor Kinase 3 (GRK3) in Olfaction -- Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcium Imaging -- Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates -- Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations -- Numerical Models and In Vitro Assays to Study Odorant Receptors -- High-Throughput Odorant Receptor Deorphanization Via pS6-Immunoprecipitation and mRNA Profiling -- Patch-Clamp Recordings from Mouse Olfactory Sensory Neurons -- In Vivo Electrophysiological Recordings of Olfactory Receptor Neuron Units and Electro-Olfactograms in Anesthetized Rats -- Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction -- Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ -- Long-term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multi-Electrode in Acute Brain Slice Preparations -- Multi-Site Recording of Local Field Potentials in Awake, Free-Moving Mice -- In Vivo Two-Photon Imaging of the Olfactory System in Insects -- Approaches for Assessing Olfaction in Children with Autism Spectrum Disorder -- Methods in Rodent Chemosensory Cognition -- Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs -- Tracking Odorant Plumes -- Generative Biophysical Modeling of Dynamical Networks in the Olfactory System -- Behavioral Assays in the Study of Olfaction: A Practical Guide 
653 |a Neuroscience 
653 |a Neurosciences 
700 1 |a Antunes, Gabriela  |e [editor] 
041 0 7 |a eng  |2 ISO 639-2 
989 |b Springer  |a Springer eBooks 2005- 
490 0 |a Methods in Molecular Biology 
028 5 0 |a 10.1007/978-1-4939-8609-5 
856 4 0 |u https://doi.org/10.1007/978-1-4939-8609-5?nosfx=y  |x Verlag  |3 Volltext 
082 0 |a 612.8 
520 |a This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to achieve a unifying comprehension of the olfactory system. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and multidisciplinary, Olfactory Receptors: Methods and Protocols serves to benefit not only those who are beginning in the field of olfactory receptors, but also those who are already working in the field and that want to learn another advanced techniques