Mouse Oocyte Development Methods and Protocols
This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2018, 2018
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| Edition: | 1st ed. 2018 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse
- In vitro Culture of Mouse Oocytes for Meiotic Maturation
- In vitro Maturation and Culture of Human Oocytes
- Pronuclear Transfer in Human Oocytes
- In vitro Fertilization of Mouse Oocyte
- Profiling Maternal mRNA Translation during Oocyte Development
- Transcriptome Profiling of Single Mouse Oocytes
- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation
- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation
- Triple-colour Live Imaging of Mouse Oocytes
- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes
- Manipulating Cohesin Levels in Live Mouse Oocytes
- Optogenetic Manipulation of Mouse Oocytes
- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin
- Photoactivation of Actin in Mouse Oocyte
- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes
- Micropipette Aspiration of Oocytes to Assess Cortical Tension