Handbook of ELISPOT Methods and Protocols
This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research,...
| Other Authors: | |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2018, 2018
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| Edition: | 3rd ed. 2018 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Challenges in Developing Protein Secretion Assays at a Single-Cell Level
- To Count of Not to Count: ELISPOT Evaluation Revisited
- Essential Controls for ELISpot Assay
- Automatic Search of Spots and Color Classification in ELISPOT Assay
- Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages
- Detection of Cross-Reactive B Cells Using the FluoroSpot Assay
- Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations
- Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays
- Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm
- Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite
- B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells
- Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT
- ELISPOT-Based “Multi-Color FluoroSpot” to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections
- Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses
- Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses
- Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection
- Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins
- Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines
- Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay