Bacterial Regulatory RNA Methods and Protocols
This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2018, 2018
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| Edition: | 1st ed. 2018 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Workflow for a Computational Analysis of an sRNA Candidate in Bacteria
- Molecular Phylogenomics for the Identification and Characterization of trans-encoded Small Non-coding RNA Families
- Bioinformatic Approach for Prediction of CsrA/RsmA-regulating small RNAs in Bacteria
- Host-pathogen Transcriptomics by Dual RNA-seq
- Identification of New Bacterial Small RNA Targets using MS2 Affinity Purification Coupled to RNA Sequencing
- Assessment of External Guide Sequences’ (EGS) Efficiency as Inducers of RNase P-mediated Cleavage of mRNA Target Molecules
- Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-dependent Termination of Transcription in vitro
- A Method to Map Changes in Bacterial Surface Composition Induced by Regulatory RNAs
- Fluorescence-based Methods for Characterizing RNA interactions in vivo
- Mutational Analysis of sRNA-mRNA Base Pairing by Electrophoretic Mobility Shift Assay
- An Integrated Cell-free Assay to Study TranslationRegulation by Small Bacterial Non-coding RNAs
- Quantitative Super-resolution Imaging of Small RNAs in Bacterial Cells
- Extraction and Analysis of RNA Isolated from Pure Bacteria—Derived outer Membrane Vesicles
- Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in Escherichia coli
- High-resolution, High-throughput Analysis of Hfq Binding Sites using UV-crosslinking and Analysis of cDNA (CRAC)
- Producing Hfq/Sm Proteins and sRNAs for Structural and Biophysical Studies of Ribonucleoprotein Assembly
- Single Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing
- Techniques to Analyse sRNA-protein Cofactor Self-assembly in vitro
- Sequence-specific Affinity Chromatography of RNA-binding Proteins from Bacterial Cells
- Identification of Small RNA-protein Partners in Plant Symbiotic Bacteria
- A Modular Genetic System for High-throughput Profiling and Engineering of Multi-target Small RNAs.