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180102 ||| eng |
| 020 |
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|a 9781493975532
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| 100 |
1 |
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|a Boheler, Kenneth R.
|e [editor]
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| 245 |
0 |
0 |
|a The Surfaceome
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Kenneth R. Boheler, Rebekah L. Gundry
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| 250 |
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|a 1st ed. 2018
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| 260 |
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|a New York, NY
|b Humana
|c 2018, 2018
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| 300 |
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|a XII, 348 p. 68 illus., 47 illus. in color
|b online resource
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| 505 |
0 |
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|a Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens -- Shaving” Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins --
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| 505 |
0 |
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|a Fabrication and Mechanical Properties Measurements of 3D Microtissues for the Study of Cell-Matrix Interactions.- Discovery of Surface Target Proteins Linking Drugs, Molecular Markers, Gene Regulation, Protein Networks, and Disease by Using a Web-Based Platform Targets-Search
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| 505 |
0 |
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|a Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes.- Mass Spectrometry Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and their Derivatives.- Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and its Proteome from Cells in Suspension.- Proteomic Profiling of Secreted Proteins, Exosomes, and Microvesicles in Cell Culture Conditioned Media.- Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-phosphate Receptor, from Sf9 Cells Using the Baculovirus System.- Bispecific Antibody Armed T Cells to Target Cancer Cells --
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| 505 |
0 |
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|a Immunophenotyping of Live Human Pluripotent Stem Cells by Flow Cytometry.- Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4.- NaV Channels: Assaying Biosynthesis, Trafficking, Function.- High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs).- Methods for Evaluation of Vascular Endothelial Cell Function with Transient Receptor Potential (TRP) Channel Drugs.- Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase TrkB in Neurons -- Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes.- Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells under Flow.- Generation of Induced Pluripotent Stem Cells from Patients with COL3A1 Mutations and Differentiation to Smooth Muscle Cells for ECM-Surfaceome Analyses --
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| 653 |
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|a Cell Biology
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| 653 |
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|a Cytology
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| 700 |
1 |
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|a Gundry, Rebekah L.
|e [editor]
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 490 |
0 |
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|a Methods in Molecular Biology
|
| 028 |
5 |
0 |
|a 10.1007/978-1-4939-7553-2
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4939-7553-2?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
0 |
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|a 571.6
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| 520 |
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|a This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions totheir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field
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