RNAi and Small Regulatory RNAs in Stem Cells Methods and Protocols
This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knoc...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2017, 2017
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| Edition: | 1st ed. 2017 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- RNAi Technique in Stem Cell Research: Current Status and Future Perspectives
- RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells
- Forward RNAi Screens in Human Hematopoietic Stem Cells
- Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology
- High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay
- Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
- Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells
- Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
- A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells
- RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
- CRISPR-Cas9-Mediated Gene Editing in Mouse Spermatogonial Stem Cells
- An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells
- Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
- RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells
- In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation
- A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans
- Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology
- Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs
- Using Quantitative Real-Time PCR to Detect MicroRNA Expression Profile During Embryonic Stem Cell Differentiation
- Genetic Tools for Self-Organizing Culture of Mouse Embryonic Stem Cells Via Small Regulatory RNA-Mediated Technologies, CRISPR/Cas9 and Inducible RNAi