3D Cell Culture Methods and Protocols

This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniq...

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Bibliographic Details
Other Authors: Koledova, Zuzana (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2017, 2017
Edition:1st ed. 2017
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • 3D Cell Culture: An Introduction
  • Preparation of Decellularized Biological Scaffolds for 3D Cell Culture
  • 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix
  • Hydrogel-Based In Vitro Models of Tumor Angiogenesis
  • Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels
  • Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions.oids
  • 3d="" fluid-dynamic="" models="" multiple="" myeloma
  • preparation="" three-dimensional="" full="" thickness="" skin="" equivalent
  • analysis="" breast="" cell="" invasion="" using="" an="" system
  • 3d="" the="" brain="" parenchyma="" metastasis="" interface="" metastasis
  • 3d="" neural="" in="" dual="" hydrogel="" systems
  • 3d="" micro-patterned="" hydrogels="" prepared="" by="" photomask,="" micro-needle,="" or="" soft="" lithography="" techniques
  • 3d="" niche="" engineering="" via="" two-photon="" laser="" polymerization
  • microfluidic-based="" generation="" collagen="" spheres="" investigate="" multicellular="" spheroid="" invasion
  • high-throughput="" sphere="" formation="" culture
  • high-throughput="" tumor="" recyclable="" microfluidic="" platform
  • high-throughput="" platform="" mesenchymal="" anastomosed="" microvascular="" network="" living="" capillary="" networks="" and="" endothelial="" cell-lined="" channels
  • human="" small="" airway-on-a-chip="" protocol
  • microfluidic="" bioprinting="" heterogeneous="" tissue="" constructs
  • bioprinting="" decellularized="" extracellular="" matrix="" bioink
  • bioprinting="" cartilage="" peg="" hydrogel
  • real-time="" cycle="" imaging="" melanoma
  • revealing="" ultrastructure="" morphology="" spheroids="" electron="" microscopy
  • quantitative="" phenotypic="" image="" analysis="" cultures