RNA Methylation Methods and Protocols
This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RN...
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| Format: | eBook |
| Language: | English |
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New York, NY
Springer New York
2017, 2017
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| Edition: | 1st ed. 2017 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- LC-MS Analysis of Methylated RNA
- Comparative Analysis of Ribonucleic Acid Digests (CARD) by mass Spectrometry
- Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation
- Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-seq)
- Mapping m6A at Individual-Nucleotide Resolution using Crosslinking and Immunoprecipitation (miCLIP)
- Detection and Quantification of N6-Methyladenosine in Messenger RNA by TLC
- Illustrating the Epitranscriptome at Nucleotide Resolution using Methylation-iCLIP (miCLIP)
- Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing
- Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing
- Analysis of High-Throughput RNA Bisulfite Sequencing Data
- Statistcal Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite
- High-Throughput Mapping of 2’-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
- RiboMeth-seq: Profiling of 2’-O-Me in RNA
- In Silico Identification of RNA Modifications from High-Throughput Sequencing Data using HAMR
- High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-seq)
- Transcriptome-Wide Mapping of N1-Methyladenosine Methylome
- In Vitro Assays for RNA Methyltransferase Activity
- Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo
- Methylated mRNA Nucleotides as Regulators for Ribosomal Translation
- Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA.