Thermogenic Fat Methods and Protocols
This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, a...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2017, 2017
|
| Edition: | 1st ed. 2017 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue
- Isolation of Mouse Stromal Vascular Cells for Monolayer Culture
- Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells
- Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes
- Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells
- Isolation of Immune Cells from Adipose Tissue
- Differentiation and Metabolic Interrogation of Human Adipocytes
- Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines
- Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue
- Genetic Mouse Models: The Powerful Tools to Study Fat Tissues
- Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
- Bioenergetic Analyses in Adipose Tissue
- Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry
- Isolation and Patch-Clamp of Primary Adipocytes
- In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver
- Identification and Quantification of Human Brown Adipose Tissue
- Designing 3D Adipospheres for Quantitative Metabolic Study
- Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems
- Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides