Oat Methods and Protocols

The volume provides detailed protocols that have been developed or modified exclusively for the study of oat. The topics discussed in this book are a selection of various molecular biology and biotechnology methods, such as the application of molecular markers for polymorphism analyses and cytologic...

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Bibliographic Details
Other Authors: Gasparis, Sebastian (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2017, 2017
Edition:1st ed. 2017
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Fluorescence In Situ Hybridization in Oat
  • Oat Doubled Haploids Following Maize Pollination
  • Wide Hybridization between Oat and Pearl Millet
  • Protocol for Producing Synthetic Polyploid Oats
  • Manipulation of Oat Protoplasts for Transient Expression Assays
  • Oat Anther Culture and Use of DH-Lines for Genetic Mapping
  • Agrobacterium-Mediated Transformation of Leaf Base Segments
  • Chromatographic Methods to Evaluate Nutritional Quality in Oat
  • Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry
  • Multiplex Dipstick Immunoassay for Semi-Quantitative Determination of Fusarium Mycotoxins in Oat
  • Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
  • M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm
  • Genotyping-By-Sequencing and its Application to Oat Genomic Research
  • Genome-Wide Association Analysis using R
  • de novo Transcriptome Assembly in Polyploid Species
  • Isolation of Oat (Avena sativa L.) Total Proteins and their Prolamin Fractions for 2-D Electrophoresis
  • 2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and their Prolamin Fractions
  • Selected Bioinformatic Tools and MS (MALDI-TOF, PMF) Techniques used in the Strategy for the Identification of Oat Proteins after 2-DE.