T-Cell Differentiation Methods and Protocols
This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell tr...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2017, 2017
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| Edition: | 1st ed. 2017 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- The Vast Universe of T Cell Diversity: Subsets of Memory Cells and their Differentiation
- Basic Aspects of T Helper Cell Differentiation
- FACS Analysis of Memory T lymphocytes
- Intravital Microscopy Analysis of Hepatic T cell dynamics
- Analysis of T Cell Activation by Confocal Microscopy
- Phenotypic and Functional Analysis of Antigen-specific T Cell Exhaustion
- pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel
- Differentiation of Diverse Progenies of Memory T cells from Naïve CD8+ T Cell Precursors
- Gammaretroviral Production and T Cell Transduction To Genetically Retarget Primary T Cells Against Cancer
- Measuring Telomerase Activity in Senescent Human T Cells upon Genetic Modification
- Strategies for T Helper Cell Subset Differentiation from Naïve Precursors
- Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells
- Approaches to Detect microRNA Expression in T Cell Subsets and T CellDifferentiation
- Next-generation Sequencing Analysis of Long Non-Coding RNAs in CD4+ T Cell Differentiation
- Highly Multiplexed, Single Cell Transcriptomic Analysis of T-cells by Microfluidic PCR
- Single-cell RNA Sequencing of Human T Cells
- Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry
- Developmental and Functional Assays to Study Murine and Human gd T Cells.