Drosophila Methods and Protocols
This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols, and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description...
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| Format: | eBook |
| Language: | English |
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New York, NY
Springer New York
2016, 2016
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| Edition: | 2nd ed. 2016 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Using FlyBase: A Database of Drosophila Genes and Genomes
- The GAL4 System: A Versatile System for Manipulation and Analysis of Gene Expression
- The Q-System: A Versatile Expression System for Drosophila
- Analysis of MicroRNA Function in Drosophila
- Methods for High-Throughput RNAi screening in Drosophila Cells
- A Guide to Genome-Wide In Vivo RNAi Applications in Drosophila
- Creating Heritable Mutations in Drosophila with CRISPR-Cas9
- Performing Chromophore-Assisted Laser Inactivation in Drosophila Embryos Using GFP
- deGradFP: A System to Knockdown GFP-Tagged Proteins
- Sample Preparation and Mounting of Drosophila Embryos for Multi-View Light Sheet Microscopy
- Cultivation and Live-Imaging of Drosophila Imaginal Discs
- Cultivation and Live Imaging of Drosophila Ovaries
- Segmentation and Quantitative Analysis of Epithelial Tissues
- Laser Ablation to Probe the Epithelial Mechanics in Drosophila
- Rapid Ovary Mass-isolation (ROMi) to Obtain Large Quantities of Drosophila Egg Chambers for Fluorescent In-Situ Hybridization
- Chromatin Immunoprecipitation for Analyzing Transcription Factor Binding and Histone Modifications in Drosophila
- Protocols to Study Growth and Metabolism in Drosophila
- Protocols to Study Ageing in Drosophila
- Protocols to Study Behavior in Drosophila
- Electrophysiological Recordings from Lobula Plate Tangential Cells in Drosophila
- Methods to Establish a Drosophila Cell Line