Plant Cytogenetics Methods and Protocols

This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomi...

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Bibliographic Details
Other Authors: Kianian, Shahryar F. (Editor), Kianian, Penny M. Avoles (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2016, 2016
Edition:1st ed. 2016
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • C-Banding of Plant Chromosomes
  • Chromosome Painting by GISH and Multi-Color FISH
  • Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes
  • Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homologous Chromosomes
  • Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
  • Immunolabeling and In Situ Labelling of Isolated Plant Interphase Nuclei
  • Manipulation of Homologous Chromosome Recombination in Wheat
  • Dissecting Plant Chromosomes by the Use of Ionizing Radiation
  • Optical Nano-Mapping and Analysis of Plant Genomes
  • Flow Sorting Plant Chromosomes
  • Construction of BAC Libraries from Flow-Sorted Chromosomes
  • The Chromosome Microdissection and Microcloning Technique
  • Immunolocalization on Whole Anther Chromosome Spreads for Male Meiosis
  • Mapping Recombination Initiation Sites Using Chromatin Immunoprecipitation
  • Chromatin Immunoprecipitation to Study Plant Epigenome