Plant Signal Transduction Methods and Protocols

This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, a...

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Bibliographic Details
Other Authors: Botella, Jose R. (Editor), Botella, Miguel A. (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2016, 2016
Edition:2nd ed. 2016
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Suppressor Screens in Arabidopsis
  • Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants
  • DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants
  • Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology
  • Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation
  • Culture of the Tomato Micro-Tom Cultivar in Greenhouse
  • Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry
  • A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications
  • Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function
  • Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells
  • Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins
  • Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions
  • Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana
  • Analysis of Protein-Lipid Interactions Using Purified C2 Domains
  • Assessing Kinase Activity in Plants with In-Gel Kinase Assays
  • Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins
  • Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions
  • Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus
  • Simultaneous Determination of Plant Hormones by GC-TOF-MS.