Yeast Functional Genomics Methods and Protocols

This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, cha...

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Bibliographic Details
Other Authors: Devaux, Frédéric (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2016, 2016
Edition:1st ed. 2016
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species
  • Enhancing Structural Annotation of Yeast Genomes with RNA-Seq Data
  • Pathogen Gene Expression Profiling During Infection Using a Nanostring Ncounter Platform
  • Comparative Transcriptomics in Yeasts
  • Mapping the Transcriptome-Wide Landscape of RBP Binding sites using gPAR-CLIP-seq: Experimental Procedures
  • Mapping the Transcriptome-wide Landscape of RBP Binding Sites using gPAR-CLIP-seq: Bioinformatic Analysis
  • Translation Analysis at the Genome Scale by Ribosome Profiling
  • Biotin-Genomic Run-on (Bio-GRO): A High-Resolution Method for the Analysis of Nascent Transcription in Yeast
  • Genome-wide Probing of RNA Structures in Vitro Using Nucleases and Deep Sequencing
  • Genome-wide Chromatin Immunoprecipitation in Candida albicans and other Yeasts
  • ChIPseq in Yeast Species: from Chromatin Immumoprecipitation to High-Throughput Sequencing and Bioinformatics Data Analyses
  • Systematic Determination of Transcription factor DNA-binding Specificities in Yeast
  • Generation and Analysis of Chromosomal Contact Maps of Yeast Species
  • A Versatile Procedure to Generate Genome-Wide Spatio-Temporal Program of Replication in Yeast Species
  • Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae
  • Label-Free Quantitative Proteomics in Yeast
  • Profiling of Yeast Lipids by Shotgun Lipidomics
  • Identification of Links between Cellular Pathways by Genetic Interaction Mapping (GIM)
  • On The Mapping of Epistatic Genetic Interactions in Natural Isolates: Combining Classical Genetics and Genomics
  • Experimental Evolution and Resequencing Analysis of Yeast
  • Reconstruction and Analysis of the Evolution of Modular Transcriptional Regulatory Programs using Arboretum
  • Predicting Gene and Genomic Regulation in Saccharomyces cerevisiae, using the YEASTRACT Database: A Step By Step Guided Analysis