Post-Transcriptional Gene Regulation

This volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level. Post-Transcriptional Gene Regulation, Second Edition is organized in six sections describing bioinformatics approaches for studying post-transcriptional regul...

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Bibliographic Details
Other Authors: Dassi, Erik (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2016, 2016
Edition:2nd ed. 2016
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression
  • A Computational Approach for the Discovery of Protein-RNA Networks
  • Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells
  • Studying the Translatome with Polysome Profiling
  • Exploring Ribosome Positioning on Translating Transcripts with Ribosome Profiling
  • Studying Isoform-Specific Recruitment to Polyribosomes with Frac-Seq
  • Use of the Pbutr Reporter System for Scalable Analysis of 3' UTR-Mediated Gene Regulation
  • Comprehensive Identification of RNA-Binding Proteins by RNA Interactome Capture
  • Identifying RBP Targets with RIP-Seq
  • PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites
  • Profiling the Binding Sites of RNA-Binding Proteins with Nucleotide Resolution Using Iclip
  • A Pipeline for PAR-CLIP Data Analysis
  • Capture and Identification Of Mirna Targets By Biotin Pulldown And RNA-Seq
  • Identification of Mirna-Target RNA Interactions Using CLASH
  • Genome-Wide Analysis of A-To-I RNA Editing
  • Nucleotide-Level Profiling Of M5c RNA Methylation
  • Probing N6-Methyladenosine (M6a) RNA Modification in Total RNA with SCARLET
  • Genome-Wide Identification of Alternative Polyadenylation Events Using 3’T-Fill
  • Genome-Wide Profiling Of Alternative Translation Initiation Sites
  • Genome-Wide Study Of Mrna Isoform Half-Lives
  • Visualizing Mrna Dynamics in Live Neurons and Brain Tissues
  • Single Molecule Live-Cell Visualization of Pre-Mrna Splicing