Cartilage Tissue Engineering Methods and Protocols

This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bi...

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Bibliographic Details
Other Authors: Doran, Pauline M. (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2015, 2015
Edition:1st ed. 2015
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Cartilage Tissue Engineering: What Have We Learned in Practice?
  • Human Foetal and Adult Chondrocytes
  • Mesenchymal Stem Cells Derived from Human Bone Marrow
  • Mesenchymal Stem Cells Derived from Human Adipose Tissue
  • Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors
  • Differentiation of Human Induced Pluripotent Stem Cells to Chondrocytes
  • Gene Transfer and Gene Silencing in Stem Cells to Promote Chondrogenesis
  • Hydrogels with Tunable Properties
  • Decellularized Extracellular Matrix Scaffolds for Cartilage Regeneration
  • Use of Interim Scaffolding and Neotissue Development to Produce a Scaffold-Free Living Hyaline Cartilage Graft
  • Bioprinted Scaffolds for Cartilage Tissue Engineering
  • Scaffolds for Controlled Release of Cartilage Growth Factors
  • Nanostructured Capsules for Cartilage Tissue Engineering
  • Stratified Scaffolds for Osteochondral Tissue Engineering
  • Mechanobioreactors for Cartilage Tissue Engineering
  • Shear and Compression Bioreactor for Cartilage Synthesis
  • Microbioreactors for Cartilage Tissue Engineering
  • Transplantation of Tissue-Engineered Cartilage in an Animal Model (Xenograft and Autograft): Construct Validation
  • Proteomic Analysis of Engineered Cartilage
  • Mechanical Testing of Cartilage Constructs