Cell Cycle Oscillators Methods and Protocols

This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments i...

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Bibliographic Details
Other Authors: Coutts, Amanda S. (Editor), Weston, Louise (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2016, 2016
Edition:1st ed. 2016
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Cell Cycle Control: A System of Interlinking Oscillators
  • Model Organisms for Studying the Cell Cycle
  • Role of Computational Modelling in Understanding Cell Cycle Oscillators
  • E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene Expression
  • Cell Synchronization of Mouse Embryonic Fibroblasts
  • Cell Cycle Synchronisation in Xenopus Egg Extracts
  • Elutriation for Cell Cycle Synchronization in Fission Yeast
  • Spatiotemporal Investigation of Phosphorylation Events during Cell Cycle Progression
  • Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence
  • Building a Synthetic Transcriptional Oscillator
  • Time Series Simulations and Bifurcation Analyses of a Model of Mitotic Oscillations in Xenopus Cell-free Extracts
  • A Computational Method for Identifying Yeast Cell Cycle Transcription Factors
  • Measuring Activity and Specificity of Protein Phosphatases
  • Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an in vitro Cdk1 Targeting Assay to Study the Regulation of the g-Tubulin Complex
  • Cell Cycle Synchronisation Using a Microfluidic Synchroniser for Fission Yeast Cells
  • Detection of Protein-Protein Interactions in Tobacco BY-2 cells Using BimolecularFluorescence Complementation
  • Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic Bypass
  • Measuring APC/C Dependent Ubiquitylation in vitro
  • Using the Fly-FUCCI System for the Live Analysis of Cell Cycle Dynamics in Cultured Drosophila cells
  • Imaging Cell Cycle Phases and Transitions of LivingCells From Yeast to Woman
  • Measurement of Cdk1/cyclin B Kinase Activity by Specific Antibodies and Western Blotting
  • The use of SNAP Labeling to Study Cell Cycle Oscillatory Proteins