VEGF Signaling Methods and Protocols
This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clini...
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| Format: | eBook |
| Language: | English |
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New York, NY
Springer New York
2015, 2015
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| Edition: | 1st ed. 2015 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- VEGF Splicing and the Role of VEGF Splice Variants: From Physiological-Pathological Conditions to Specific Pre-mRNA Splicing
- Detection and Quantification of VEGF Isoforms by ELISA
- Quantitation of Circulating Neuropilin-1 in Human, Monkey, Mouse and Rat Sera by ELISA
- Detection and Quantification of Vascular Endothelial Growth Factor Receptor Tyrosine Kinases in Primary Human Endothelial Cells
- Induction of VEGF Secretion in Cardiomyocytes by Mechanical Stretch
- Chromatin Immunoprecipitation Assay: Examining the Interaction of NFkB with the VEGF Promoter
- An Overview of VEGF-Mediated Signal Transduction
- Identification of Receptor Tyrosine Kinase Inhibitors using Cell Surface Biotinylation and Affinity Isolation
- Analysis of VEGF-Mediated ERK5 Activity in Endothelial Cells
- In Vitro Angiogenesis Assays
- Chemotactic Migration of Endothelial Cells Towards VEGF-A165
- Vasculogenesis and Angiogenesis in VEGF Receptor-1 Deficient Mice
- The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis
- VEGF Gene Transfer to the Utero-Placental Circulation of Pregnant Guinea Pigs to Enhance Fetal Growth
- VEGF Gene Transfer to the Utero-placental Circulation of Pregnant Sheep to Enhance Fetal Growth
- Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System