Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single...
| Main Authors: | , , , |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Cham
Springer International Publishing
2015, 2015
|
| Edition: | 1st ed. 2015 |
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Introduction
- Health and Safety Considerations
- Sample Collection and Storage
- Low-Cost DNA Extraction
- PCR Amplification for Low-Cost Mutation Discovery
- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
- Example Data
- Conclusions.