Membrane Trafficking Second Edition

This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular...

Full description

Bibliographic Details
Other Authors: Tang, Bor Luen (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2015, 2015
Edition:2nd ed. 2015
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Intracellular Parcel Service: Current Issues in Intracellular Membrane Trafficking
  • In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides
  • Import of Proteins into Isolated Yeast Mitochondria
  • Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae
  • Fractionation of Plasmodium-infected Human Red Blood Cells to Study Protein Trafficking
  • Investigating Signalling Processes in Membrane Trafficking
  • Recruitment of Coat Proteins to Liposomes and Peptidoliposomes
  • A β-lactamase Based Assay to Measure Surface Expression of Membrane Proteins
  • Cell-free Reconstitution of Multivesicular Body (MVB) Cargo Sorting
  • The Analysis of the Biogenesis of Lipid Droplets by Examining Rab40c Associating With Lipid Droplets
  • Analysis of Conventional and Unconventional Trafficking of CFTR and Other Membrane Proteins
  • Assessing Mammalian Autophagy
  • Expression of Functional Myc-tagged Conserved Oligomeric Golgi (COG) Subcomplexes in Mammalian Cells
  • Molecular and Cellular Characterization of GCC185, a Tethering Protein of the trans-Golgi Network
  • Visualizing Toll-like Receptor-dependent Phagosomal Dynamics in Murine Dendritic Cells Using Live Cell Microscopy
  • Understanding of Complex Protein Interactions with Respect to Anchorage Independence
  • Application of Flow Cytometry to Analyse Intracellular Location and Trafficking of Cargo in Cell Populations
  • Approaches to Analyze the Role of Rab GTPases in Endocytic Trafficking of Epidermal Growth Factor Receptor (EGFR)
  • Does Super Resolution Fluorescence Microscopy Obsolete Previous Microscopic Approaches to Protein Co-localization?
  • Bimolecular Fluorescence Complementation (BiFC) Technique in Yeast Saccharomyces cerevisiae and Mammalian Cells
  • Microscopic and Spectroscopic Techniques to Investigate Lipid Droplet Formation and Turnover in Yeast
  • Image-based Identification of Nuclear Export Inhibitors From Natural Products.-Correlative Video-light-electron Microscopy of Mobile Organelles
  • Live Cell Imaging of Endosomal Trafficking in Fungi
  • Quantitative Analysis of Transferrin Cycling by Automated Fluorescence Microscopy
  • Identification of Factors Regulating MET Receptor Endocytosis by High Throughput siRNA Screening
  • Large-scale Analysis of Membrane Transport in Yeast Using Invertase Reporters
  • RNAi Screens for Genes Involved in Golgi Glycosylation
  • Proteomics Analyses of an Unique Bi-lobed Structure in Trypanosoma brucei
  • Application of the Proximity-dependent Assay and Fluorescence Imaging Approaches to Study Viral Entry Pathways