Cryopreservation and Freeze-Drying Protocols

This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as...

Full description

Main Author: Wolkers, Willem F.
Corporate Author: SpringerLink (Online service)
Other Authors: Oldenhof, Harriëtte (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2015, 2015
Edition:3rd ed. 2015
Series:Methods in Molecular Biology, Methods and Protocols
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Summary:This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens
Physical Description:XIII, 509 p. 90 illus., 35 illus. in color online resource
ISBN:9781493921935