Plasmodesmata Methods and Protocols
Plasmodesmata: Methods and Protocols provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular tra...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2015, 2015
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| Edition: | 1st ed. 2015 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Plasmodesmata: Channels for Intercellular Signaling During Plant Growth and Development
- Plasmodesmata: Channels for Viruses on the Move
- Imaging Plasmodesmata with High Resolution Scanning Electron Microscopy
- Preparative Methods for Imaging Plasmodesmata at Super-Resolution
- Isolation of Plasmodesmata from Arabidopsis Suspension Culture Cells
- Immunofluorescence Detection of Callose Deposition around Plasmodesmata Sites
- Imaging Callose at Plasmodesmata Using Aniline Blue: Quantitative Confocal Microscopy
- Localization of Fluorescently-Tagged Protein to Plasmodesmata by Correlative Light and Electron Microscopy
- Quantification of Plant Cell Coupling with Live-Cell Microscopy
- Drop-ANd-See: A Simple, Real-Time, and Non-Invasive Technique for Assaying Plasmodesmal Permeability
- Mapping Symplasmic Fields at the Shoot Apical Meristem Using Iontophoresis and Membrane Potential Measurements
- Analysis of the Conductivity of Plasmodesmata by Microinjection
- Investigating Plasmodesmata Genetics with Virus-Induced Gene Silencing and an Agrobacterium-Mediated GFP Movement Assay
- Probing Plasmodesmata Function with Biochemical Inhibitors
- GAL4 Transactivation-Based Assay for the Detection of Selective Intercellular Protein Movement
- Techniques for Assessing the Effects of Pharmacological Inhibitors on Intercellular Protein Movement
- Probing Protein Targeting to Plasmodesmata Using Fluorescence Recovery after Photo-Bleaching
- The Tracking of Intercellular Small RNA Movement
- Analysis of the Role of Myosins in Targeting Proteins to Plasmodesmata
- Pumilio-Based RNA In Vivo Imaging
- In Vivo RNA Labeling Using MS2