In Vitro Transcription and Translation Protocols
Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has mad...
| Other Authors: | |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
1995, 1995
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| Edition: | 1st ed. 1995 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Transcription In Vitro Using Bacteriophage RNA Polymerases
- Subtraction Hybridization cDNA Libraries
- Quantitative Measurement of mRNA Using the RNase Protection Assay
- Assembly and Transcription of Chromatin Templates Using RNA Polymerase III
- Antisense Affinity Depletion of RNP Particles
- In Vitro mRNA Editing Using S100 Extracts
- An In Vitro Transcription Assay for Probing Drug-DNA Interactions During Active Transcription of DNA
- In Vitro Reconstitution of Progesterone-Dependent RNA Transcription in Nuclear Extracts of Human Breast Carcinoma Cells
- Plastid In Vitro Transcription
- Microinjection of In Vitro Transcribed RNA and Antisense Oligonucleotides in Mouse Oocytes and Early Embryos to Study the Gain-and Loss-of-Function of Genes
- mRNA Translation in Xenopus Oocytes
- Transcription System Using a HeLa Cell Mitochondrial Lysate
- The Xenopus Egg Extract Translation System
- In Vitro Translation Using Rabbit Reticulocyte Lysate
- An In Vitro Transcription System from BmN Cells of the Silkworm, Bombyx mori
- Translation Using a Wheat-Germ Extract
- In Vitro Transcription and Translation in a Cell-Free System from Clostridium tetani
- Preparation and Use of E. coli S-30 Extracts
- Posttranslational Transport and In Thylakoido Integration of Plastid Protein
- Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro
- Structure-Function studies based on in vitro expression
- ?-Like Plastid Transcription Factors
- Preparation of HeLa Nuclear Extracts
- Purification of the human TATA-Binding Protein, TBP
- DNA-Binding studies using in vitro synthesized myb proteins
- DNase i footprinting using PCR-generated end-labeled DNA probes
- Gene Isolation by Screening ?gt11 Expression Libraries with DNA Binding-Site Probes.-Transcriptional activation analysis by the Chloramphenicol Acetyl Transferase (CAT) enzyme assay