Polyadenylation Methods and Protocols
In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation. Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexit...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2014, 2014
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| Edition: | 1st ed. 2014 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- 3’ end Formation and Regulation of Eukaryotic mRNAs
- Poly(A) Polymerase-based Poly(A) Length assay
- Using Klenow Mediated Extension to Measure Poly(A)-tail Length and Position in the Transcriptome
- Detection of Uridylated mRNAs
- Cytoplasmic Polyadenylation Assays
- In vitro Analysis of Transcriptional Activators and Polyadenylation
- Fluorescence Detection of Polyadenylation Reaction through the Coordination of Adensoine2-coralyne-adensoine2
- Small molecule recognition of poly(A).-The Stem-loop Luciferase assay for Polyadenylation (SLAP) Method for Determining CstF-64-dependent Polyadenylation Activity
- Accurate Mapping of Cleavage and Polyadenylation Sites by 3' Region Extraction and Deep Sequencing
- Polyadenylation Site Prediction using PolyA-iEP Method
- Detecting alternative polyadenylation from Microarray Data
- Quantitative Polyadenylation Site Mapping with Single-molecule Direct RNA Sequencing
- In vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes.-microRNA-mediated Deadenylation in a Mammalian Cell-free System
- Quantitative Analysis of Deadenylation-independent mRNA Decay by a Modified MBRACE Assay
- RHAPA: A New Method to Quantify Alternative Polyadenylation
- Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq Strategy to Profile poly(A+) RNA
- Global and Quantitative Profiling of Polyadenylated RNAs using PAS-seq
- High-throughput Sequencing of RNA Isolated by Crosslinking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs
- Polyadenylation in Bacteria and Organelles
- In vivo Analysis of Polyadenylation in Prokaryotes
- Polyadenylation of RNA in E. coli: RNA Polymerase-associated (rA)n-synthetic Activities
- Analysis of the Human Mitochondrial Transcriptome using Directional Deep sequencing and Parallel Analysis of RNA Ends
- Measurement of Mitochondrial RNA Stability by Metabolic Labeling of Transcripts with 4-Thiouridine
- Deadenylation and its Regulation in Eukaryotic Cells
- Assaying mRNA Deadenylation in vitro
- Assaying mRNA Deadenylation in vivo