Gene Correction Methods and Protocols
Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies ut...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2014, 2014
|
| Edition: | 1st ed. 2014 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- RecTEPsy Mediated Recombineering in Pseudomonas syringae
- Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila
- Multiple Genetic Manipulations of DT40 Cell Line
- Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombination
- Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cells
- Small Fragment Homologous Replacement (SFHR): Sequence-Specific Modification of Fenomic DNA in Eukaryotic Cells by Small DNA Fragments
- Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction
- Triplex-Mediated Genome Targeting and Editing
- Targeting piggyBac Transposon Integrations in the Human Genome
- Gene Targeting in Human Induced Pluripotent Stem Cells with Adenoviral Vectors
- Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-Associated Virus
- Lentiviral Vectors Encoding Zinc-Finger Nucleases Specific for the Model Target Locus HPRT1
- Designing and Testing the Activities of TAL Effector Nucleases
- A Bacterial One-Hybrid System to Isolate Homing Endonuclease Variants with Altered DNA Target Specificities
- Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correction
- CRISPR-Cas Mediated Targeted Genome Editing in Human Cells
- RNA-Guided Genome Editing of Mammalian Cells
- Nuclease-Mediated Double Strand Break (DSB) Enhancement of Small Fragment Homologous Recombination (SFHR) Gene Modification in Human Induced Pluripotent Stem Cells (hiPSCs)
- AAV-Mediated Gene Editing via Double-Strand Break Repair
- Genetic Modification Stimulated by the Induction of a Site-Specific Break Distant from the Locus of Correction in Haploid and Diploid Yeast Saccharomyces cerevisiae
- A Southern Blot Protocol to Detect Chimeric Nuclease-Mediated Gene Repair
- High-Throughput Cellular Screening of Engineered Nuclease Activity Using the Single-Strand Annealing Assay and Luciferase Reporter
- An Unbiased Method for Detection of Genome-Wide Off Target Effects in Cell Lines Treated with Zinc Finger Nucleases
- Identification of Off-Target Cleavage Sites of Zinc Finger Nucleases and TAL Effector Nucleases Using Predictive Models
- Method for Retinal Gene Repair in Neonatal Mouse
- In utero Delivery of Oligodeoxynucleotides for Gene Correction
- Portal Vein Delivery of Viral Vectors for Gene Therapy for Hemophilia
- Gene Correction of Induced Pluripotent Stem Cells Derived from a Murine Model of X-Linked Chronic Granulomatous Disorder
- Efficient Transduction of Hematopoietic Stem Cells and its Potential for Gene Correction of Hematopoietic Diseases