Skeletal Development and Repair Methods and Protocols
Skeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation, as well as, the source of tissues and cells for in vitro culture and analyses. Chapters...
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2014, 2014
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| Edition: | 1st ed. 2014 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Overview of Skeletal Development
- Overview of Skeletal Repair (Fracture Healing and Its Assessment)
- Generation of Closed Transverse Fractures in Small Animals
- The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool
- Surgical Induction of Posttraumatic Osteoarthirits
- A Mouse Model of Flexor Tendon Repair
- Cartilage Explant Cultures
- Renal Capsule Transplantations to Assay Skeletal Angiogenesis
- Whole Mount Skeletal Staining
- Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens
- Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto’s Film Method (2012)
- Demineralized Murine Skeletal Histology
- Beta-Galactosidase Staining in the Skeleton
- Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues
- Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections
- Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections
- Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections
- TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis
- Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme
- Isolation and Culture of Murine Primary Chondrocytes
- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo
- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts
- RANKL-BasedOsteoclastogenic Assays from Murine Bone Marrow Cells
- Hematopoietic Stem Cell Cultures and Assays