The Biochemistry of the Nucleic Acids
When the first edition of this book was published in 1950, it predated the publication of the double-helical structure of DNA by three years. It is not, therefore, surprizing that nothing of the original book remains in the current edition. Indeed, such is the pace of change in the field of nucleic...
| Main Authors: | , , |
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| Format: | eBook |
| Language: | English |
| Published: |
Dordrecht
Springer Netherlands
1992, 1992
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| Edition: | 11th ed. 1992 |
| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- 1 Introduction
- 2 The structure of the nucleic acids
- 3 Genomes of eukaryotes, bacteria and viruses: chromosome organization
- 4 Degradation and modification of nucleic acids
- 5 The metabolism of nucleotides
- 6 Replication of DNA
- 7 Repair, recombination and rearrangement of DNA
- 8 The arrangement of genes
- 9 RNA biosynthesis
- 10 Control of transcription
- 11 Processing of RNA transcripts and its control
- 12 The translation of mRNA: protein synthesis
- Appendix: methods of studying nucleic acids
- A.1 Occurrence and chemical analysis
- A.1.1 Chemical determination of nucleic acids in tissues
- A.1.2 Analysis of base composition and nearest-neighbour frequency
- A.1.3 Estimation of the molecular weight of DNA
- A.2 Isolation and separation of nucleic acids
- A.2.1 Isolation and separation of DNA
- A.2.2 Isolation and separation of RNA
- A.3 Hybridization of nucleic acids
- A.4 Methods of labelling nucleic acids
- A.4.1 General labelling methods
- A.4.2 End-labelling methods
- A.5 Determination of nucleic acid sequences
- A.5.1 Determination of DNA sequences
- A.5.2 Determination of RNA sequences
- A.6 Restriction mapping of DNA
- A.7 The polymerase chain reaction
- A.8 Cloning DNA
- A.8.1 The principles
- A.8.2 The major cloning vectors
- A.8.3 cDNA cloning
- A.8.4 Genomic cloning
- A.9 Analysis and manipulation of cloned DNA
- A.9.1 Mapping of RNA transcripts
- A.9.2 Identification of regions of DNA that interact with proteins
- A.9.3 Expression
- A.10 Chemical synthesis of oligonucleotides
- A.11 Cell-free systems for transcription and translation
- A.12 The use of inhibitors in the study of gene expression
- References