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140122 ||| eng |
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|a 9783642839627
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100 |
1 |
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|a Hewitt, Godfrey M.
|e [editor]
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245 |
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|a Molecular Techniques in Taxonomy
|h Elektronische Ressource
|c edited by Godfrey M. Hewitt, Andrew W.B. Johnston, J.Peter W. Young
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250 |
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|a 1st ed. 1991
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260 |
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|a Berlin, Heidelberg
|b Springer Berlin Heidelberg
|c 1991, 1991
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300 |
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|a X, 410 p
|b online resource
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|a A Prologue -- Past and Future of Taxonomy -- Overviews -- Taxonomy: an Essential Key to Evolutionary Biology -- Variation at the DNA Level: Something for Everyone -- Molecular Systematics at the Species Boundary: Exploiting Conserved and Variable Regions of the Mitochondrial Genome of Animals via Direct Sequencing from Amplified DNA -- Ribosomal RNA Phylogenies -- Evaluating Gene Versus Genome Evolution -- DNA and Higher Plant Systematics: Some Examples From the Legumes -- DNA-DNA Hybridization: Principles and Results -- Satellite DNA -- DNA Fingerprinting -- The Statistical Interpretation of Hypervariable DNAs -- Case Studies -- A Multidimensional Approach to the Evolution and Systematics of Dolichopoda Cave Crickets -- Sperm and Evolution in Drosophila: Morphological and Molecular Aspects -- Episodic Evolutionary Change in Local Populations -- Searching for Speciation Genes in the Species Pair Drosophila Mojavensis and D. Arizonae -- Colonizing Species of Drosophila -- Molecular Taxonomy in the Control of West African Onchocerciasis -- Protocols -- DNA Protocols for Plants -- Prepartion and Visualization of Mitochondrial DNA for RFLP Analysis -- Hybridization of DNA Probes to Filterbound DNA Using the “DIG-DNA Labeling and Detection System Nonradioactive” (Boehringer Mannheim) -- DNA Fingerprinting -- The Polymerase Chain Reaction: DNA Extraction and Amplification -- Protocols for 4-Cutter Blots and PCR Sequencing -- Ribosomal RNA Sequencing -- Quantitative DNA:DNA Hybridization and Hydroxyapatite Elution -- A Protocol for the TEACL Method of DNA-DNA Hybridization -- List of Participants
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653 |
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|a Cell Biology
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653 |
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|a Zoology
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653 |
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|a Cytology
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653 |
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|a Botany
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653 |
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|a Plant Science
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700 |
1 |
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|a Johnston, Andrew W.B.
|e [editor]
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700 |
1 |
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|a Young, J.Peter W.
|e [editor]
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041 |
0 |
7 |
|a eng
|2 ISO 639-2
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989 |
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|b SBA
|a Springer Book Archives -2004
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490 |
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|a Nato ASI Subseries H:, Cell Biology
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028 |
5 |
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|a 10.1007/978-3-642-83962-7
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856 |
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|u https://doi.org/10.1007/978-3-642-83962-7?nosfx=y
|x Verlag
|3 Volltext
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|a 590
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|a Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing
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