Molecular Techniques in Taxonomy

Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1...

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Bibliographic Details
Other Authors: Hewitt, Godfrey M. (Editor), Johnston, Andrew W.B. (Editor), Young, J.Peter W. (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 1991, 1991
Edition:1st ed. 1991
Series:Nato ASI Subseries H:, Cell Biology
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
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260 |a Berlin, Heidelberg  |b Springer Berlin Heidelberg  |c 1991, 1991 
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505 0 |a A Prologue -- Past and Future of Taxonomy -- Overviews -- Taxonomy: an Essential Key to Evolutionary Biology -- Variation at the DNA Level: Something for Everyone -- Molecular Systematics at the Species Boundary: Exploiting Conserved and Variable Regions of the Mitochondrial Genome of Animals via Direct Sequencing from Amplified DNA -- Ribosomal RNA Phylogenies -- Evaluating Gene Versus Genome Evolution -- DNA and Higher Plant Systematics: Some Examples From the Legumes -- DNA-DNA Hybridization: Principles and Results -- Satellite DNA -- DNA Fingerprinting -- The Statistical Interpretation of Hypervariable DNAs -- Case Studies -- A Multidimensional Approach to the Evolution and Systematics of Dolichopoda Cave Crickets -- Sperm and Evolution in Drosophila: Morphological and Molecular Aspects -- Episodic Evolutionary Change in Local Populations -- Searching for Speciation Genes in the Species Pair Drosophila Mojavensis and D. Arizonae -- Colonizing Species of Drosophila -- Molecular Taxonomy in the Control of West African Onchocerciasis -- Protocols -- DNA Protocols for Plants -- Prepartion and Visualization of Mitochondrial DNA for RFLP Analysis -- Hybridization of DNA Probes to Filterbound DNA Using the “DIG-DNA Labeling and Detection System Nonradioactive” (Boehringer Mannheim) -- DNA Fingerprinting -- The Polymerase Chain Reaction: DNA Extraction and Amplification -- Protocols for 4-Cutter Blots and PCR Sequencing -- Ribosomal RNA Sequencing -- Quantitative DNA:DNA Hybridization and Hydroxyapatite Elution -- A Protocol for the TEACL Method of DNA-DNA Hybridization -- List of Participants 
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653 |a Cytology 
653 |a Botany 
653 |a Plant Science 
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700 1 |a Young, J.Peter W.  |e [editor] 
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520 |a Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing