Nonconventional Yeasts in Biotechnology A Handbook

Nonconventional yeasts - all yeasts other than S. cerevisiae and S. pombe - are attracting increasing attention in basic research and biotechnological applications. Due to their exceptional metabolic pathways, they have been used in various biotechnological processes for producing foods or food addi...

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Bibliographic Details
Main Author: Wolf, Klaus
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 1996, 1996
Edition:1st ed. 1996
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • 5 Genetic Techniques
  • 6 Chromosomal DNA
  • 7 Genes and Genetic Markers
  • 8 Vector Systems
  • 9 Heterologous Gene Expression
  • 10 The Amylolytic System
  • 11 Industrial Applications
  • References
  • 5 Kluyveromyces lactis
  • 1 History of Kluyveromyces lactis Research
  • 2 Physiology
  • 3 Growth Media
  • 4 Available Strains
  • 5 Genetic Techniques
  • 6 Chromosomal DNA
  • 7 Genes and Genetic Markers
  • 8 K. lactis Genes vs. S. cerevisiae Genes
  • 9 Regulation of Carbon Metabolism
  • 10 Mitochondria
  • 11 A Few Notes on Biochemical Procedures
  • 12 Plasmids
  • 13 Vector Systems
  • 14 Transformation Procedures
  • 15 K. lactis for Industrial Application
  • References
  • 6 Pichia pastoris
  • 1 History of Pichia pastoris
  • 2 Growth and Storage
  • 3 Available Strains
  • 4 Genetic Techniques
  • 5 Fermentation Process
  • 6 Transformation
  • 7 Induction of Protein Expression
  • 8 Analysis of Protein Expression
  • 9 Vectors
  • 10 Optimization of Protein Expression
  • 3 Cytology and Morphology of Candida maltosa
  • 4 Genetics and Molecular Biology of Candida maltosa
  • 5 Host-Vector Systems for Candida maltosa
  • 6 Potential Biotechnological Application of Candida maltosa
  • References
  • 13 Trichosporon
  • 1 History of Trichosporon Research
  • 2 Available Strains and Mutant Collections
  • 3 Media for Different Purposes
  • 4 Conservation of Strains
  • 5 Genetic Techniques
  • 6 Biochemical Techniques
  • 7 Molecular Techniques
  • 8 Specific Biochemical Properties of Trichosporon Yeasts
  • 9 Trichosporon Cell Biology: Staining of Nuclei
  • 10 Applications of Trichosporon Yeasts
  • References
  • 1 Principles and Methods Used in Yeast Classification, and an Overview of Currently Accepted Yeast Genera
  • 1 Introduction
  • 2 Some Principles of Yeast Taxonomy
  • 3 Trends in the Systematics of Yeasts
  • 4 Phylogeny
  • 5 Methods
  • 6 Overview of Yeast Genera
  • 7 Appendix
  • References
  • 2 Protoplast Fusion of Yeasts
  • 1 Introduction
  • 2 Transfer of Cytoplasmic Genes
  • 3 Production of Polyploid Strains
  • 4 Fusion of Strains with Identical Mating Type
  • 5 Establishment of Parasexual Genetic Systems
  • 6 Fusion of Strains Belonging to Different Species or Genera
  • 7 Practical Recommendations
  • 8 Analysis of the Fusants
  • 9 Additional Protocols
  • 10 Concluding Remarks
  • References
  • 3 Electrophoretic Karyotyping of Yeasts
  • 1 Introduction and Theory
  • 2 Fields of Application
  • 3 Practical Recommendations
  • References
  • 4 Schwanniomyces occidentalis
  • 1 History of Schwanniomyces occidentalis Research
  • 2 Physiology
  • 3 Media
  • 4 Available Strains
  • 9 Heterologous Gene Expression
  • 10 Peroxisomal Biogenesis
  • 11 Applied Aspects
  • 12 Concluding Remarks
  • References
  • 10 Yarrowia lipolytica
  • 1 History of Yarrowia lipolytica Research
  • 2 Physiology/Biochemistry/Cell Structure
  • 3 Life Cycle
  • 4 Genetic and Molecular Data
  • 5 Transformation, Vectors, and Expression Systems
  • 6 Media and Culture Conditions
  • 7 Genetic Techniques
  • 8 Methods of Cell Biology (Structural Studies)
  • 9 Methods of Molecular Biology/Gene Technology
  • References
  • 11 Arxula adeninivorans
  • 1 History of Arxula adeninivorans Research
  • 2 Physiology and Biochemical Procedures
  • 3 Growth Media
  • 4 Available Strains and Preservation Methods
  • 5 Parasexual Genetics
  • 6 Chromosomal DNA
  • 7 Mitochondrial DNA
  • 8 Transformation System
  • 9 Expression of Heterologous Genes in A. adeninivorans
  • References
  • 12 Candida maltosa
  • 1 History and Taxonomy of Candida maltosa
  • 2 Physiology and Biochemistry of Candida maltosa
  • 11 Miscellaneous Procedures
  • References
  • 7 Pichia guilliermondii
  • 1 History of Pichia guilliermondii Research
  • 2 Physiology
  • 3 Available Strains
  • 4 Genetic Techniques
  • 5 Chromosomes, Genes, and Genetic Markers
  • 6 DNA Isolation and Transformation
  • 7 Biochemical Genetics
  • 8 Biotechnological Applications
  • 9 Concluding Remarks
  • References
  • 8 Pichia methanolica (Pichia pinus MH4)
  • 1 History of Pichia methanolica Research
  • 2 Physiology
  • 3 Available Strains
  • 4 Genetic Techniques
  • 5 Chromosomes
  • 6 Genes and Genetic Markers
  • 7 Transformation
  • 8 Genetic Control of Mating
  • 9 Biochemical Genetics of Purine Biosynthesis
  • 10 Genetic Control of Methanol Metabolism
  • 11 Biotechnological Applications
  • References
  • 9 Hansenula polymorpha (Pichia angusta)
  • 1 History of Hansenula polymorpha Research
  • 2 Physiology
  • 3 Media
  • 4 Available Strains
  • 5 Genetic Techniques
  • 6 Chromosomes
  • 7 Genes and Genetic Markers
  • 8 Vector Systems