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140122 ||| eng |
| 020 |
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|a 9783642790690
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| 100 |
1 |
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|a Rothe, Gunter M.
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| 245 |
0 |
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|a Electrophoresis of Enzymes
|h Elektronische Ressource
|b Laboratory Methods
|c by Gunter M. Rothe
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| 250 |
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|a 1st ed. 1994
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| 260 |
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|a Berlin, Heidelberg
|b Springer Berlin Heidelberg
|c 1994, 1994
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| 300 |
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|a XII, 307 p
|b online resource
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| 505 |
0 |
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|a 1 Introduction -- 1.1 Enzyme Classification -- 1.2 Enzyme Subunit Composition -- 1.3 Isozyme Classification -- 1.4 References -- 2 Extraction of Enzymes from Tissues, Cells and Cell-Organelles -- 2.1 Methods to Extract Enzymes from Microorganisms -- 2.2 Methods to Extract Enzymes from Animal Soft Tissue -- 2.3 Differential Extraction of Cytosolic and Mitochondrial Enzymes from Animal Soft Tissue -- 2.4 Extraction of Insects -- 2.5 Extraction of Plant Tissues -- 2.6 Concentration of Diluted Enzyme Extracts -- 2.7 Mammalian Blood -- 2.8 Pancreatic Islets -- 2.9 Isolation of Subcellular Organelles -- 2.10 Catalytic Activities of Enzymes Forming a Common Metabolic Sequence -- 2.11 References -- 3 Methods for Separating Native Enzymes -- 3.1 General Considerations -- 3.2 Cellulose Acetate Electrophoresis -- 3.3 Starch Gel Electrophoresis -- 3.4 References -- 3.5 Analytical Polyacrylamide Gel Electrophoresis for Separating Native Enzymes -- 3.6 2-D-Electrophoresis under Non-Denaturing Conditions -- 3.7 References -- 4 Sodium Dodecylsulphate Electrophoresis -- 4.1 Homogeneous Buffer Systems -- 4.2 Discontinuous Buffer Systems -- 4.3 Gradient Gel Systems -- 4.4 Enzyme Visualization Following SDS-Electrophoresis -- 4.5 References -- 5 Chemistry of Enzyme Visualization -- 5.1 Methods for Visualizing Oxidative Enzymes -- 5.2 Methods for Visualizing Transferases -- 5.3 Methods for Visualizing Hydrolases -- 5.4 Methods for Visualizing Lyases -- 5.5 Methods for Localizing Isomerases and Ligases -- 5.6 Visualization of Enzymes by Different Methods -- 5.7 References -- 6 A Compilation of Protocols to Visualize Enzymes -- 6.1 Index to Enzyme Recipes and Separation Systems -- 6.2 Protocols to Visualize Enzymes Following Electrophoretic Separation -- 6.3 References -- 7 Data Evaluation in Population Genetics and Evolution -- 7.1 Allozymes as Gene Markers -- 7.2 Population Genetic Measures -- 7.3 Calculation of Dendrograms -- 7.4 References
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| 653 |
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|a Food Science
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| 653 |
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|a Analytical chemistry
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| 653 |
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|a Analytical Chemistry
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| 653 |
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|a Chemistry, Technical
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| 653 |
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|a Biochemistry
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| 653 |
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|a Food science
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| 653 |
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|a Industrial Chemistry
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 490 |
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|a Springer Lab Manuals
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| 028 |
5 |
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|a 10.1007/978-3-642-79069-0
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| 856 |
4 |
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|u https://doi.org/10.1007/978-3-642-79069-0?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 572
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