Electron Probe Microanalysis Applications in Biology and Medicine
The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg
Springer Berlin Heidelberg
1989, 1989
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| Edition: | 1st ed. 1989 |
| Series: | Springer Series in Biophysics
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Quantitative X-ray elemental mapping of dynamic physiologic events in skeletal muscle
- Single isolated cardiac myocytes frozen during voltage-camp pulses: A technique for correlating X-ray micro-analysis data on calcium distribution with calcium inward current in the same cell
- X-ray microanalysis of fast exocytotic processes
- 4. Medical Application
- Electron probe microanalysis in pathology
- Microprobe analysis in medicine — present practice and future trends
- The history of electron probe microanalysis in biology
- 1. Specimen Preparation
- Specimen preparation and other limitations in quantitative electron probe X-ray microanalysis using ultrathin sections
- Freeze-substitution and low temperature embedding for analytical electron microscopy
- Ensuring the validity of results in biological X-ray microanalysis
- 2. Analytical Techniques
- a) X-ray microanalysis
- The subcellular accumulation of toxic heavy metals: Qualitative and quantitative X-ray microanalysis
- X-ray microanalysis of cryosections using image analysis
- Electron probe X-ray microanalysis in the silkmoth antenna — problems with quantification in ultrathin cryosections
- b) Electron energy loss spectroscopy
- Progress in electron energy loss spectroscopic imaging and analysing biological specimens with a field emission scanning transmission electron microscope
- Application of parallel-detection electron energy loss spectroscopy in biology
- Resin based standards for biological energy dispersive X-ray and electron energy loss microanalysis
- Imaging and microanalysis by electron spectroscopy
- 3. Biological Applications
- a) Intracellular element localization
- Application of X-ray microanalysis and electron energy loss spectroscopy to studies of secretory cell biology
- X-ray microanalysis of freshly isolated cells in suspension
- X-ray microanalysis and free calcium measurements in cultured neonatal rat ventricular myocytes
- b) Epithelial transport
- 1/um thick frozen hydrated/dried sections for analysing pericellular environment in transport epithelia; New results from old data
- Distribution of ions and water in epithelial cells and tissues
- Characterization of electrolyte transport mechanisms and compartments by the use of the markers Rb and Br.-Electron probe analysis of transport properties of cultured cells
- c) Dynamic processes