Plant Protoplasts and Genetic Engineering I

Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in t...

Full description

Bibliographic Details
Main Author: Bajaj, Y. P. S.
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 1989, 1989
Edition:1st ed. 1989
Series:Biotechnology in Agriculture and Forestry
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Section I Isolation Culture and Preservation of Protoplasts
  • I.1 Recent Advances in the Isolation and Culture of Protoplasts and Their Implications in Crop Improvement
  • I.2 Enzymes for the Isolation of Protoplasts
  • I.3 Viability of Plant Protoplasts
  • I.4 Agarose Plating of Protoplasts and Its Applications
  • I.5 Immobilization of Plant Protoplasts
  • I.6 Cryopreservation of Plant Protoplasts
  • Section II Regeneration of Plants from Protoplasts
  • II.1 Regeneration of Plants from Rice Protoplasts
  • II.2 Isolation and Culture of Potato Protoplasts
  • II.3 Isolation and Culture of Citrus Protoplasts
  • II.4 Regeneration of Plants from Linum Protoplasts
  • II.5 Isolation and Culture of Glycine Protoplasts
  • II.6 Regeneration of Plants from Cabbage (Brassica oleracea var. capitata) Protoplasts
  • II.7 Regeneration of Plants from Chicory (Chicorium intybus L.) Protoplasts
  • II.8 Regeneration of Plants from Protoplasts of Lettuce and its Wild Species
  • II.9 Regeneration of Plants from Protoplasts of Butterbur (Petasites japonicus, Fr. Schmidt)
  • II.10 Isolation and Culture of Orchid Protoplasts
  • II.11 Regeneration of Plants from Protoplasts of Salpiglossis
  • II.12 Isolation and Culture of Protoplasts of Forest Tree Species
  • Section III Protoplast Fusion and Somatic Hybridization
  • III.1 Fusion of Plant Protoplasts — Techniques
  • III.2 Ultrastructural Studies of Plant Protoplast Fusion
  • III.3 Symmetric Versus Asymmetric Fusion Combinations in Higher Plants
  • III.4 Somatic Hybridization in Tomato
  • III.5 Lycopersicon and Petunia Somatic Hybrids
  • III.6 Somatic Hybridization in Hyoscyamus and Nicotiana
  • III.7 Medicago Protoplasts: Fusion, Culture and Plant Regeneration
  • III.8 Somatic Hybridization Between Moricandia and Brassica
  • III.9 Somatic Hybridization in Trifolium.-III.10 Somatic Hybridization in Rice × Soybean