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| 008 |
140122 ||| eng |
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|a 9783642603457
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| 100 |
1 |
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|a Rogan, Michael T.
|e [editor]
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| 245 |
0 |
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|a Analytical Parasitology
|h Elektronische Ressource
|c edited by Michael T. Rogan
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| 250 |
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|a 1st ed. 1997
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| 260 |
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|a Berlin, Heidelberg
|b Springer Berlin Heidelberg
|c 1997, 1997
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| 300 |
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|a XVII, 365 p. 28 illus
|b online resource
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|a 10 Immunological Analysis of Parasite Molecules -- 10.1 Introduction -- 10.2 Parasite Extracts -- 10.3 Production of Antibodies to Parasite Molecules -- 10.4 Enzyme Linked Immunosorbent Assays (ELISA) -- 10.5 Immunoblotting -- 10.6 Affinity Chromatography -- References
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| 505 |
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|a 8 Studies of the Surface Properties, Lipophilic Proteins and Metabolism of Parasites by the Use of Fluorescent and “Caged” Compounds -- 8.1 Introduction -- 8.2 Incorporation of Fluorescent Compounds into Membranes -- 8.3 Using Fluorescent Techniques to Measure Membrane Fluidity -- 8.4 Distribution of Actin -- 8.5 Assessment of Parasite Membrane Damage -- 8.6 Localisation of Probes in Organelles -- 8.7 Measurement of pH at the Surface of Parasites and Intracellular pH -- 8.8 The Use of Caged Compounds -- 8.9 Use of Fluorescent Probes in the Analysis of Parasite Proteins -- References -- 9 Detection and Identification of Parasite Surface Carbohydrates by Lectins -- 9.1 Introduction -- 9.2 Lectin-Mediated Trypanosomatid Parasite Agglutination -- 9.3 Fluorescein-Isothiocyanate (FITC)-Conjugated Lectins -- 9.4 Peroxidase-Labelled Lectins -- 9.5 Biotinylated Lectins -- 9.6 Fixatives and Enzymatic Treatments -- 9.7 Histological Sections -- 9.8 Controls -- References --
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|a 4.7 Concluding Remarks -- References -- 5 Parasite Proteinases -- 5.1 Introduction -- 5.2 Sample Preparation -- 5.3 Substrates and Proteinase Assays -- 5.4 Proteinase Inhibitors -- 5.5 Electrophoretic Methods -- 5.6 Purification Methods -- 5.7 Endogenous Inhibitors -- 5.8 Final Comments -- References -- 6 Neurobiology of Helminth Parasites -- 6.1 Introduction -- 6.2 Immunocytochemical Localisation of Neuroactive Substances in Helminth Parasites -- 6.3 Characterisation and Purification of Neuropeptides -- 6.4 Physiological Action of Transmitters and Drug Assessment In Vitro -- References -- 7 Electron Microscopy in Parasitology -- 7.1 Introduction -- 7.2 Preparation for Transmission Electron Microscopy (TEM) -- 7.3 Cytochemistry and Staining Protocol in TEM -- 7.4 Preparation for Scanning Electron Microscopy (SEM) -- 7.5 Immunogold-Labelling Techniques -- References --
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|a 1 Biochemical Pathways in Parasites -- 1.1 Introduction -- 1.2 Preparation of Enzyme Fractions -- 1.3 Enzyme Assays -- 1.4 Metabolite Measurements -- 1.5 Use of Inhibitors -- References -- 2 Electrophoresis of Parasite Proteins -- 2.1 Introduction -- 2.2 Lysis of Parasites and Extraction of Proteins -- 2.3 SDS-PAGE -- 2.4 Modifications of Standard Method -- 2.5 Applications of SDS-PAGE to the Characterisation of Individual Polypeptides -- References -- 3 Molecular Techniques in Analytical Parasitology -- 3.1Introduction -- 3.2 Isolation of Parasite Nucleic Acid -- 3.3 Gel Electrophoresis and Blotting of Nucleic Acid -- 3.4 Labelling and Hybridisation of DNA Probes -- References -- 4 The Production and Analysis of Helminth Excretory-Secretory (ES) Products -- 4.1 Introduction -- 4.2 Collection of Excretory-Secretory (ES) Products -- 4.3 Acetylcholinesterase -- 4.4 Proteolytic Enzymes -- 4.5 Anticoagulants, Blood Coagulation Assays -- 4.6 Potential Immunomodulators --
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| 653 |
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|a Cell Biology
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| 653 |
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|a Cytology
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| 653 |
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|a Immunology
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| 653 |
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|a Biochemistry
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| 653 |
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|a Medical Microbiology
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| 653 |
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|a Medical microbiology
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 490 |
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|a Springer Lab Manuals
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| 028 |
5 |
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|a 10.1007/978-3-642-60345-7
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| 856 |
4 |
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|u https://doi.org/10.1007/978-3-642-60345-7?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 571.6
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| 520 |
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|a Parasites are of global significance in terms of human an animal health and research is constantly targeted at controlling such infections. In order for this to be effective, detailed analyses of the biology of each species, particularly at the molecular level, must be carried out to promote the development of new therapeutic or diagnostic approaches. This laboratory manual, with detailed background information and practical protocols, will be a useful guide for researchers engaged in many areas of parasitology. Most techniques described can be applied to both helminthic and protozoan parasites, although protocols relevant to individual species are also included. It is largely recommended for postgraduate and postdoctoral scientists and provides procedures for some basic techniques in immunological, microscopical, and molecular analyses along with more specialized schemes to give a multidisciplinary approach to experimental parasitology. Parasite infections are very widespread, nevertheless effective therapeuticals are not yet available. The analysis of the life cycle and the parasite host interactions at the molecular level may help in the search of the "Achilles heel" of a parasite and thus promote the development of new therapeutic approaches. Parasite molecules such as surface antigens, excretory proteins or metabolic enzymes may serve as targets for new diagnostics tests, chemo- or immunotherapeutics or even as candidate vaccine
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