Techniques in Animal Cytogenetics
A better "casting" could not be conceived. The authors of this book are gold smiths on the subject. I have followed their work since their "entry" into cyto genetics and I have a high esteem for them. I consider it an honour to be asked to write the preface of their opus. Paul...
| Other Authors: | , , |
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| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Principles and Practice
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- VIII.5 In situ hybridisation to chromosomes in suspension
- S Solutions for chromosome staining and banding techniques
- M Miscellaneous
- L Solutions for lampbrush chromosomes
- F Solutions for flow and slit scan cytometry
- H Solutions for in situ hybridization
- CM Culture media
- SS Stock solutions
- B Buffer solutions
- V Solutions for in vivo treatments
- References
- I Preparation of Chromosome Spreads
- I.1 Cell culture techniques
- I.2 Preparation of chromosomes in prophase or prometaphase
- I.3 Direct techniques and very short term development cultures
- I.4 Preparation of chromosome spreads
- I.5 Staining techniques of chromosome spreads
- II Chromosome Banding Techniques
- II. 1 Introduction
- II.2 Techniques based on DNA structure
- II.3 Banding techniques based on DNA replication
- II.4 Techniques of chromosome differentiation based on DNA base composition
- II.5 Heterochromatin staining
- II.6 Staining of nucleolar organiser regions NOR
- II.7 Techniques of sequential banding
- III In Situ Hybridisation Techniques
- III.1 Introduction
- III.2 Methods
- III.3 Remarks on other applications of in situ hybridization
- IV Methods of Germ Cells Study
- IV.1 Meiosis in male
- IV.2 Meiosis in the mammalian female
- IV.3 Study of the spermatozoa by interspecific in vitro fertilisation (insemination)
- V The Lampbrush Chromosomes of Amphibians
- V.1Introduction: The basis of lampbrush chromosomes mapping
- V.2 Technique for the preparation of lampbrush chromosomes for light microscopy
- V.3 Preparation of lampbrush chromosomes for electron microscopy
- V.4 Preparation of mitotic chromosomes
- V.5 Analysis of lampbrush chromosomes
- V.6 The importance of the lampbrush chromosomes
- VI Techniques for the study of Drosophila Chromosomes
- VI.1 Mitotic chromosomes
- VI.2 Polythene chromosomes
- VII Techniques for the stuey of Interphase Nucleus
- VII.1 Sex chromatin examination
- VII.2 Released chromatin (Chromatin halo)
- VII.3 In situ hybridisation of interphasic nuclei
- VIII Application of Flow Cytometry and Slit-Scan Fluorometry in Analysis and Sorting of Mammalian Chromosomes
- VIII.1 Introduction
- VIII.2 Principles of the flow cytometry
- VIII.3 Methods of chromosome preparation and staining
- VIII.4 Measurements and evaluation in flow cytometry