Immobilized Cells

Immobilized cells have been studied widely in the last few decades, and an enormous number of papers have been published on this topic. For many processes the complex physiology in a heterogeneous environment is now becoming clear. In addition it has been shown that in many processes it is more effi...

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Bibliographic Details
Other Authors: Wijffels, Rene H. (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 2001, 2001
Edition:1st ed. 2001
Series:Springer Lab Manuals
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
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505 0 |a 1 Characterization of Immobilized Cells; Introduction -- Immobilization -- 2 Description of the Support Material -- 3 Description of the Immobilization Procedures -- 4 Measurement of Density, Particle Size and Shape of Support -- 5 Mechanical Stability of the Support -- 6 Diffusion Coefficients of Metabolites -- Kinetics -- 7 Quantity of Biomass Immobilized, Determination of Biomass Concentration -- 8 Kinetics of the Suspended Cells -- 9 Diffusion Limitation -- 10 Micro-Electrodes -- 11 Biomass Gradients -- 12 NMR and Immobilized Cells -- Engineering -- 13 Immobilization at Large Scale by Dispersion -- 14 Immobilization at Large Scale with the Resonance Nozzle Technique -- 15 External Mass Transfer -- 16 Liquid Fluidization of Gel-Bead Particles -- 17 Gradients in Liquid, Gas or Solid Fractions -- 18 Support Material Stability at the Process Conditions Used -- Cases -- 19 Immobilized Cells in Food Technology: Storage Stability and Sensitivity to Contamination -- 20 Immobilized Cells in Bioremediation -- 2 Plasmid Stability in Immobilized Cells -- 22 Immobilization for High-Throughput Screening 
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520 |a Immobilized cells have been studied widely in the last few decades, and an enormous number of papers have been published on this topic. For many processes the complex physiology in a heterogeneous environment is now becoming clear. In addition it has been shown that in many processes it is more efficient to use immobilized cells than suspended cells. The contributions in this volume by scientists from various disciplines in academia, industry and research institutes cover the path from basic physiological research to actual application. One of the goals was to extract guidelines for characterizing immobilized cells in view of process design and application. It is important to recognize that research on immobilized metabolizing cells should eventually lead, whenever possible, to successful implementation of such catalysts in industrial processes, and so one of the aims of using these guidelines is to ensure that research is effective in achieving this objective. A structure was chosen in which general aspects of immobilization as well as kinetics and engineering aspects were covered. Besides laboratory methods, some simple calculation procedures have been presented in this lab manual. The combination of the practical methods with the calculations provide a tool for research with immobilized cells. Finally, the manual concludes with some recent promising and practical cases of immobilized cells as examples of potential applications