Monoclonal Antibodies and Functional Cell Lines Progress and Applications

This volume serves as a follow-up to our previous book, MonoclonalAntibodies­ Hybridomas: A New Dimension in Biological Analyses. We continue the theme of monoclonal antibodies and their applications, attempting to cover some of the areas not covered in the previous volume. We again include an appen...

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Bibliographic Details
Other Authors: Kennett, Roger H. (Editor)
Format: eBook
Language:English
Published: New York, NY Springer US 1984, 1984
Edition:1st ed. 1984
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • V Developing Areas of Biotechnology
  • 11 Production of Human Monoclonal Antibodies
  • 12 Monoclonal Antibodies and Molecular Genetics: Oncogenes and Oncogene Products
  • 13 Functional Murine T-Cell Clones
  • Appendix Additional Methods for Production and Characterization of Monoclonal Antibodies and Continuous Cell Lines
  • Zdenka L. Jonak and Roger H. Kennett
  • Fusion of Nonadherent Human Cell Lines
  • Kenneth E. Truitt, James W. Larrick, and James W. Larrick
  • ELISA for Immunoglobulin Subclass Determination
  • Anselmo Otero and Roger H. Kennett
  • Immunofiltration: A Rapid Screening Assay for Detection of Antibodies Directed against Cell Surface Antigens
  • Elliott K. Main, Mary Kate Hart, and Darcy B. Wilson
  • Binding Inhibition by Cell Extracts
  • Lois Alterman Lampson
  • Immunoblots and Autoradiography Used to Analyze the Proteins Recognized by Monoclonal Antibodies
  • Cheryl A. Fisher and Lois Alterman Lampson
  • Peroxidase as an Alternative to Radiolabels for Immunoblotting Assays
  • Gerd G. Maul
  • The Use of Streptavidin in the Detection of Monoclonal Antibodies
  • Rosemary J. Versteegen and Christine Clark
  • Solid Phase Identification and Molecular Weight Determination of Cell Membrane Antigens with Monoclonal Antibodies
  • Roberto L. Ceriani
  • Antigen Localization in Tissue Sections
  • Kathleen B. Bechtol
  • Microscopic Analysis of Monoclonal Antibody Binding to Agarose-Embedded Cell Lines
  • Lois Alterman Lampson, James P. Whelan, and Carol J. Lawton
  • Derivation of T-Cell Clones
  • Andrew L. Glasebrook and Frank W. Fitch
  • Methods for Transfection of Human DNA into Primary Mouse Lymphocytes and NIH/3T3 Mouse Fibroblasts
  • Zdenka L. Jonak and Roger H. Kennett
  • Cell Lines for Hybridoma Formation and References to Other Methods
  • I Introduction
  • 1 Introduction: Reflections on Nine Years of Monoclonal Antibodies from Hybridomas
  • II Characterization of the Detailed Antigenic Structure of Macromolecules
  • 2 Monoclonal Antibodies Directed against the Nicotinic Acetylcholine Receptor
  • 3 Monoclonal Antibodies to Enzymes
  • 4 Monoclonal Antibodies Directed to Cell-Surface Carbohydrates
  • III Detection and Isolation of Antigens in Complex Biological Systems
  • 5 Adhesion-Related Integral Membrane Glycoproteins Identified by Monoclonal Antibodies
  • 6 Monoclonal Antibodies to Cytoskeletal Proteins
  • 7 Molecular Bases of Neuronal Individuality: Lessons from Anatomical and Biochemical Studies with Monoclonal Antibodies
  • IV Toward Practical Applications in Medicine
  • 8 Monoclonal Antibodies to Human Lymphocytes: Clinical Application in the Therapy of Leukemia
  • 9 Monoclonal Antibodies to Human Neuroblastoma Cells and Other Solid Tumors
  • 10 Monoclonal Antibodies and Immunoparasitology