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Instrumental Analysis in the Biological Sciences

Instrumental techniques of analysis have now moved from the confines of the chemistry laboratory to form an indispensable part of the analytical armoury of many workers involved in the biological sciences. It is now quite out of the question to considcr a laboratory dealing with the analysis of biol...

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Bibliographic Details
Main Author: Gordon, M. H.
Format: eBook
Language:English
Published: New York, NY Springer US 1987, 1987
Edition:1st ed. 1987
Subjects:
Humanities And Social Sciences
Humanities
Social Sciences
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • 8.3 Qualitative analysis
  • 8.4 Quantitative analysis
  • 8.5 Instrumentation
  • 8.7 Attenuated total reflectance
  • 8.8 Near-infrared reflectance analysis
  • References
  • 9 Nuclear magnetic resonance spectroscopy
  • 9.1 Introduction
  • 9.2 Principles
  • 9.3 Pulse NMR spectrometer
  • 9.4 Chemical shifts
  • 9.5 Spin—spin coupling
  • 9.6 Integration
  • 9.7 Further techniques for elucidation of NMR spectra
  • 9.8 Wide-line NMR
  • 9.9 In-vivo NMR
  • References
  • 10 Electron spin resonance
  • 10.1 Principles
  • 10.2 ESR spectra
  • 10.3 ESR spectrometer
  • 10.4 Sample preparation
  • 10.5 Spin labelling
  • 10.6 Quantitative analysis
  • References
  • 11 Flame techniques
  • 11.1 Introduction
  • 11.2 Flame emission spectrometry (FES)
  • 11.3 Atomic absorption spectrometry (AAS)
  • 11.4 Applications
  • References
  • 12 Mass spectrometry
  • 12.1 Introduction
  • 12.2 Mass spectrometer
  • 12.3 Analysis of mixtures
  • 12.4 Determination of molecular structures
  • References
  • 1 Introduction to instrumental methods of analysis
  • 1.1 Precision and accuracy
  • 1.2 Speed of analysis
  • 1.3 Cost
  • 1.4 Safety
  • 1.5 Automation
  • Recommended general texts
  • 2 Liquid chromatography
  • 2.1 Introduction
  • 2.2 Theory of liquid chromatography
  • 2.3 Modes of chromatography
  • 2.4 Chromatographic techniques
  • 2.5 Sample preparation
  • References
  • 3 Gas chromatography
  • 3.1 Introduction
  • 3.2 Principles
  • 3.3 The chromatographic system
  • 3.4 GLC columns
  • 3.5 Principles of separation
  • 3.6 Stationary phases
  • 3.7 Gas—solid chromatography
  • 3.8 Detectors
  • 3.9 Sample preparation
  • 3.10 Quantification
  • References
  • 4 Electrophoresis
  • 4.1 Introduction
  • 4.2 Effect of pH on charge
  • 4.3 Techniques of electrophoresis
  • 4.4 Isotachophoresis
  • References
  • 5 Introduction to spectroscopy
  • 5.1 Spectroscopy
  • 5.2 The electromagnetic spectrum
  • 5.3 Molecular energy states
  • 5.4 Molecular transitions
  • 5.5 Quantitative analysis
  • 13 Electrochemical techniques
  • 13.1 Introduction
  • 13.2 Conductivity of solutions
  • 13.3 Voltammetry
  • 13.4 Potentiometric measurements
  • References
  • 5.6 Determination of a spectrum
  • Further reading
  • 6 UV—visible spectrophotometry
  • 6.1 Introduction
  • 6.2 Electronic energy levels
  • 6.3 Electronic transitions
  • 6.4 Qualitative analysis
  • 6.5 Quantitative analysis
  • 6.6 Calibration of spectrophotometers
  • 6.7 Sample presentation
  • 6.8 Difference spectrophotometry
  • 6.9 Spectrophotometric titrations
  • 6.10 Derivative spectrophotometry
  • 6.11 Dual-wavelength spectrophotometry
  • 6.12 Spectrophotometers and colorimeters
  • 6.13 Turbidimetry and nephelometry
  • 6.14 Colour and gloss of solid samples
  • References
  • 7 Fluorescence and phosphorescence spectrophotometry
  • 7.1 Introduction
  • 7.2 Fluorophores
  • 7.3 Excitation and emission spectra
  • 7.4 Quantitative measurements
  • 7.5 Factors affecting fluorescence spectra
  • 7.6 Instruments for fluorescence studies
  • 7.7 Applications of fluorescencespectrophotometry
  • References
  • 8 Infrared spectroscopy
  • 8.1 Introduction
  • 8.2 Molecular vibrations

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