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Chemical Mutagens Principles and Methods for Their Detection: Volume 2

The best protection against environmental mutagens is to identify them before they ever come into general use. But it is always possible that some substance will escape detection and affect a large number of persons without this being realized until later generations. This article considers ways in...

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Bibliographic Details
Other Authors: Hollaender, Alexander (Editor)
Format: eBook
Language:English
Published: New York, NY Springer US 1971, 1971
Edition:1st ed. 1971
Subjects:
Medical Genetics
Veterinary Science
Bioorganic Chemistry
Veterinary Medicine
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • 23 Human Population Monitoring
  • I. Introduction
  • II. A Classification of Mutational Effects
  • III. Criteria for a Mutation-Monitoring System
  • IV. Some Ways of Amplifying the Mutation-Detecting Power of Monitoring Systems
  • V. Monitoring for Germinal Mutations
  • A. Monitoring for Dominant Mutant Phenotypes
  • B. Biochemical Monitoring
  • VI. Summary
  • VII. References
  • Conclusion
  • Author Index
  • IV. Spermatogonial and Meiotic Chromosome Preparations
  • V. Bone Marrow Biopsy Procedure
  • VI. Discussion
  • VII. Acknowledgments
  • VIII. References
  • 15 Induction and Analysis of Gene Mutations in Mammalian Cells in Culture
  • I. Introduction
  • II. The Cell Material
  • III. Utilization of In Vivo Markers
  • IV. Detection of Recessive Mutations in Cell Cultures
  • VI. Characterization of Newly Isolated Variants
  • VII. Procedure for Mutation Induction
  • VIII. Concluding Remarks
  • IX. Acknowledgments
  • X. References
  • 16 Inducing Mutations with Chemicals in Habrobracon
  • I. Introduction
  • II. Maintenance of Habrobracon and Ephestia
  • III. Scheme for Mutational Analysis
  • IV. Application of Mutagens
  • V. Conclusions
  • VI. References
  • 17 The Detection of Mutations in Drosophila melanogaster
  • I. Introduction
  • II. Advantages of Drosophila as a Test Organism
  • III. Limitations of Drosophila as a Test Organism
  • IV. Lethal Tests
  • VII. In Vivo and in Vitro Studies
  • VIII. Specific Techniques
  • IX. Summary
  • X. References
  • 20 Specific Locus Mutation in Mice
  • I. Introduction
  • II. The Method, Its Advantages and Disadvantages
  • III. Results Obtained
  • IV. Conclusions
  • V. References
  • 21 Dominant Lethal Mutations in Mammals
  • I. Introduction
  • II. The Dominant Lethal Syndrome
  • III. The Estimation of Dominant Lethals
  • IV. The Genotypes of Dominant Lethals
  • VI. Dominant Lethals in Female Germ Cells
  • VII. General Validity of the Dominant Lethal Test
  • VIII. Synergistic Effects
  • IX. Review of Chemicals Tested as Dominant Lethal Mutagens in Mammals
  • X. Integration of the Dominant Lethal Assay and Other Mutagenicity Tests into General Toxicological Practice
  • XI. References
  • 22 TheHost-Mediated Assay, a Practical Procedure for Evaluating Potential Mutagenic Agents in Mammals
  • I. Introduction
  • II. Materials and Methods
  • III. Results
  • IV. Conclusion
  • V. References
  • of Volume 2
  • 11 Measurement of Recessive Lethal Damage Over the Entire Genome and at Two Specific Loci in the ad-3 Region of a Two-Component Heterokaryon of Neurospora Crassa
  • I. Introduction
  • II. Measurement of the Genetic Effects of Mutagenic Treatment
  • III. Characterization of Presumptive ad-3 Mutants
  • IV. Media and Chemical Solutions
  • V. References
  • 12 Aspergillus
  • I. Introduction
  • II. Life Cycle and Genetic Analysis
  • III. Mutation
  • IV. Conclusions
  • V. References
  • 13 Higher Plants
  • I. Introduction
  • II. Test Systems
  • III. Compounds Tested for Mutagenic Activity by Treatment of Seeds
  • IV. Special Techniques That Have a Higher Resolving Power
  • V. General Evaluation
  • VI. Acknowledgments
  • VII. References
  • 14 Procedures for Culturing Diploid Cells and Preparation of Meiotic Chromosomes from Dwarf Species of Hamsters
  • I. Introduction
  • II. Background Information
  • III. Handling the Diploid Cell
  • V. Tests for Recessive Visible Mutants
  • VI. Tests for Chromosomal Rearrangements
  • VII. Tests for Loss of X or Y Chromosomes
  • VIII. Tests for Dominant Lethals
  • IX. Tests for Half-Translocations
  • X. Staging of Germ Cells
  • XI. Techniques for Collecting Flies of a Desired Sex
  • XII. Review of Literature
  • XIII. Summary
  • XIV. References
  • 18 Root Tips for Studying the Effects of Chemicals on Chromosomes
  • I. The Material
  • II. Treatment of Root Tips with Chemicals
  • III. Fixation and Staining of Root Tips
  • IV. Scoring of Slides and Types of Aberration
  • V. Comparison Between the Effects of Chemicals on Chromosomes in Root-Tip Cells and in Cultured Animal Cells
  • VI. Acknowledgments
  • VII. References
  • VIII. Suggested Reading
  • 19 Cytogenetic Studies in Animals
  • I. Introduction
  • II. Experimental Design
  • III. Classification of Chromosomal Aberrations
  • IV. Localization of Chromosomal Aberrations
  • V. Timing of Chromosomal Damage
  • VI. Meiotic Studies

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