Cell Surface Receptors: A Short Course on Theory and Methods A Short Course on Theory and Methods
Cell Surface Receptors: A Short Course on Theory and Methods, Second Edition is a primer for the study of cell surface receptors. The simplified discussion of methods and their underlying principles removes the usual intimidation caused by the specialized vocabulary or sophisticated mathematics that...
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| Format: | eBook |
| Language: | English |
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New York, NY
Springer US
1996, 1996
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| Edition: | 2nd ed. 1996 |
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| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- 6 The topographical fate of ligand-receptor complexes as reflected by the properties of ligand binding to intact cells
- Biochemical approaches for discriminating between cell surface receptors versus intracellular receptor-ligand complexes
- Biochemical evidence consistent with recycling of cell surface receptors
- Assessment of rate constants for receptor turnover using a steady state mathematical analysis of intact cell radioligand binding data
- The heavy amino acid density-shift technique for quantitating receptor synthesis and turnover
- Summary
- 1 Historical perspective
- The origin of the receptor concept
- Mass action law and occupancy theory
- The concept of efficacy
- The concept of spare receptors
- Rate theory
- Allosteric theory
- Operational models of pharmacological agonism
- Summary
- 2 Methods for characterization of receptors based on receptor-mediated responses in tissue or intact cell preparations
- Characterization of receptor specificity
- Generalizations regarding the determination of equilibrium dissociation constants (KD values) for receptor-ligand interactions in intact tissue preparations
- Determination of KD values for receptor-agonist interactions, KDA
- Determination of KD values for receptor-partial agonist interactions, KDP
- Determination of KD values for receptor-antagonist interactions, KDB
- Summary
- 3 Identification of receptors using direct radioligand binding techniques
- Methods—data generation
- Choice of a radioligand
- The incubation
- Separation of bound from free radioligand
- Criteria expected for binding of D to the physiological receptor, R
- Data obtained to establish the criteria for a physiologically relevant receptor
- Summary
- 4 Complex binding phenomena
- Mathematical descriptions of complex binding phenomena
- Computer-assisted analysis of complex binding phenomena
- Analysis of receptor subtypes
- Independent data consistent with the existence of receptor subtypes
- Receptor affinity states
- The ternary complex model (TCM) and expansions of the TCM
- Thermodynamic parameters of receptor-ligand interactions
- Summary
- 5 The preparation and study of detergent-solubilized receptors
- General properties of biological membranes and detergent micelles
- Choice of a biological detergent
- Solubilizing receptors from biological membranes
- Methods for analysis of detergent-solubilized receptors
- Summary