Aquatic Microbial Ecology Biochemical and Molecular Approaches

Aquatic microbial ecology, a growing interdisciplinary field, has become increasingly compartmentalized in recent years. The aim of this volume is to propose a framework for biochemical and molecular approaches, which are employed ever more widely in studies of aquatic microbial communities and ecos...

Full description

Bibliographic Details
Other Authors: Overbeck, Jürgen (Editor), Chrost, Ryszard J. (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 1990, 1990
Edition:1st ed. 1990
Series:Brock Springer Series in Contemporary Bioscience
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • 3.8 Significance of ectoenzymesin aquatic environments
  • References
  • 4 Aspects of Aquatic Microbial Carbon Metabolism: Regulation of Phosphoenolpyruvate Carboxylase
  • 4.1 Introduction
  • 4.2 Basic strategies of biochemical adaptation to the environment
  • 4.3 Anaplerotic reactions
  • 4.4 Complexity of CO2 dark uptake
  • 4.5 Phosphoenolpyruvate carboxylase
  • 4.6 Assays, preparation and kinetic properties of phoshoenolpyruvate carboxylase
  • 4.7 Regulation of phosphoenolpyruvate carboxylase by effectors
  • 4.8 Phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase
  • 4.9 Concluding remarks: biochemical approaches at the ecosystem level
  • References
  • 5 Approaches to Bacterial Population Dynamics
  • 5.1 Introduction
  • 5.2 Numerical taxonomic approaches
  • 5.3 Immunological approaches
  • 5.4 Molecular approaches
  • 5.5 Isoenzymes and genetic diversity
  • 5.6 Future prospects
  • References
  • 6 RNA Chemotaxonomy of Bacterial Isolates and Natural Microbial Communities
  • 6.1 Ecological relevance of bacterial taxonomy
  • 6.2 Systematics of bacteria based on nucleic acids analysis
  • 6.3 The concept of RNA chemotaxonomy
  • 6.4 Methodology for the analysis of pure cultures by RNA profiles
  • 6.5 General results of RNA profiles from pure cultures
  • 6.6 Identification of bacterial isolates by RNA chemotaxonomy
  • 6.7 Analysis of mixed bacterial cultures
  • 6.8 Analysis of natural bacterial communities
  • 6.9 Conclusions and future developments
  • References
  • 7 Plasmids and Their Role in Natural Aquatic Bacterial Communities
  • 7.1 Introduction
  • 7.2 Ecogenetics: a new concept of microbial ecology
  • 7.3 Plasmids in natural bacterial communities of some aquatic habitats
  • 7.4 Open questions
  • 7.5 Molecular techniques
  • 7.6 Future ecogenetics
  • References
  • 1 Introduction: Aims, Problems, and Solutions in Aquatic Microbial Ecology
  • 1.1 The aims of microbial ecology
  • 1.2 Novel solutions to “old” problems
  • References
  • 2 Origin, Composition and Microbial Utilization of Dissolved Organic Matter
  • 2.1 Introduction
  • 2.2 Origin and fate of dissolved organic matter
  • 2.3 Concentration and characteristics of dissolved organic matter
  • 2.4 Dissolved organic matter released by phytoplankton
  • 2.5 Glycolic acid: a model compound of algal release
  • 2.6 Dissolved free amino acids
  • 2.7 Dissolved free carbohydrates
  • 2.8 Polyphenols
  • 2.9 Conclusions
  • References
  • 3 Microbial Ectoenzymes in Aquatic Environments
  • 3.1 Introduction
  • 3.2 Definition of an ectoenzyme
  • 3.3 Association of ectoenzymes with microbial cells
  • 3.4 Ectoenzyme secretion
  • 3.5 Molecular control of ectoenzyme synthesis
  • 3.6 Environmental control of ectoenzyme synthesis and activity in aquatic ecosystems
  • 3.7 Methods for assaying ectoenzymes