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140122 ||| eng |
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|a 9781461202479
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| 100 |
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|a Martin, Bernice M.
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| 245 |
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|a Tissue Culture Techniques
|h Elektronische Ressource
|b An Introduction
|c by Bernice M. Martin
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| 250 |
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|a 1st ed. 1994
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| 260 |
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|a Boston, MA
|b Birkhäuser
|c 1994, 1994
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| 300 |
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|a 260 p
|b online resource
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| 505 |
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|a 1 Introduction -- 2 Sterility -- Aseptic Technique -- Sterilization Methods -- Quality Control of Sterilization -- Suggested Readings -- Exercises -- 3 Routine Cell Culture -- Feeding Schedules and Media Components -- Subcultivation -- Cell Enumeration and Cell Viability -- Putting Routine Methods to Work -- Detecting and Disposing of Contamination -- Troubleshooting -- Safety -- Suggested Readings -- Problem Set -- Exercises -- 4 Experiments in Culture -- Alterations of the Media -- Substrata -- Altering the Environment -- Problem Set -- Exercises -- 5 Primary Cell Culture -- Isolation -- Characterization -- Exercise -- 6 Cell Preservation -- Freezing the Cells -- Thawing the Cells -- Problem Set -- 7 Cell Cloning -- Isolation -- Assisting Clonal Survival -- Problem Set -- 8 Culture Changes -- The Loss of Differentiated Phenotype -- Immortality and Aging -- Cell Transformation -- Transfection (Gene Transfer) -- Suggested Readings -- Problem Set -- Exercises -- 9 Information for New Cell Studies -- Searching in the Literature -- Using Advertising and Catalogs -- References, Bibliographies, and Databases -- Appendices -- A The Cell Cycle -- Suggested Readings -- B Media and Salt Solutions -- Media -- Salt Solutions -- C Vendors -- D Glossary -- E Answers to Problem Sets and Exercises
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| 653 |
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|a Family medicine
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| 653 |
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|a General Practice and Family Medicine
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| 041 |
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|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 028 |
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|a 10.1007/978-1-4612-0247-9
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| 856 |
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|u https://doi.org/10.1007/978-1-4612-0247-9?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 610
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| 520 |
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|a . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions • water as a reagent· Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells • Common enzyme solutions • Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • Particle counter • Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi • Fungi •Mycoplasma • Viruses • Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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| 520 |
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|a 101 Coatingplasticware with solutions • Alterations with polymers • Using cells to coat the plasticware • Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes • Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection • Enzymatic dissociation methods• Nonenzymatic isolation • Purification of cell suspensions • Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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| 520 |
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|a 73 Inadequate cell growth • Recurrent contamination • When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards • Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum • Treatments of serum • Plasma-derived serum • Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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| 520 |
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|a ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations • Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat • Radiation • Toxic gas • Filtration • Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . .
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