Confocal Microscopy for Biologists
There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new flu...
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Springer US
2004, 2004
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| Edition: | 1st ed. 2004 |
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| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Visitech Confocal Microscopes
- 1. What Is Confocal Microscopy?
- 2. Understanding Microscopy
- 3. Confocal Microscopy Hardware
- 4. Image Collection
- 5. Digital Images In Microscopy
- 6. Imaging Software
- 7. Presentation And Publication
- 8. What Is Fluorescence?
- 9. Fluorescent Probes
- 10. Confocal Microscopy Techniques
- 11. Fluorescence Immunolabelling..
- 12. Imaging Live Cells
- 13. The Internet
- 14. Technical Supplies
- 15. Further Reading
- Appendix 1: Confocal Microscopes
- Laser Spot Scanning Confocal Microscopes
- Nipkow Spinning Disk Confocal Microscopes
- Nipkow Disk / Micro-lens Array Confocal Microscopes
- A. Bio-Rad Cell Science Division
- Bio-Rad Instruments
- Radiance Series of Confocal Microscopes
- Components of the Radiance Confocal Microscopes
- Radiance Scan Head
- Radiance Instrument Control Unit (ICU)
- Changing Optical Filters and Dichroic mirrors
- Radiance 2100 Rainbow
- Sels (Signal Enhancing Lens)
- Photon Counting
- Computer and Software
- Mrc Series of Confocal Microscopes
- Components of the Mrc-1024 Confocal Microscope
- Mrc-1024 Scan Head
- Scan Head Alignment
- Optical Filter Blocks
- Emission Filter Wheels
- Photomultiplier Tubes (PMT)
- Controller Box
- Computer Requirements
- Hardware set up for specific imaging methods
- Dual and Triple labelling — T 1 and T2a Filter Blocks
- Live Cell Work — B1 and Open Block Filter Blocks
- Back Scatter Imaging — B1 and T 1 Filter Blocks
- Trouble Shooting (Mrc And Radiance)
- Bio-Rad Lasersharp Software
- Image Collection Panel
- Main Control Panel
- Optics Control Panel
- Mixer Control Panel
- Carl Zeiss Microscopy
- Zeiss Confocal Microscopes
- Zeiss Lsm 510 Meta Confocal Microscope
- Components of the Zeiss 510 Meta
- Zeiss Lsm 510 Meta Scan Head
- Lasers
- Single Track and Multi Track Image Collection.-Conventional Detection Channels
- Meta Channel
- Transmission Imaging
- Zeiss Confocal Microscope Control Software
- Nikon Confocal Microscopes
- Components of the Nikon C 1 Confocal Microscope
- Nikon C1 Scan Head
- Optics/Detector Unit
- Laser Launch Unit
- Nikon C1 User Interface
- Olympus Corporation
- Olympus Confocal Microscopes
- Components of the Olympus Fluoview 1000
- Fluoview 1000 Confocal Microscope
- Sim (Simultaneous) Scanner
- Fluoview 500 Confocal Microscope
- Fluoview 500 Scan Head
- Components of the Olympus Fluoview 300 and 500
- Fluoview 300 Confocal Microscope
- Fluoview 300 Scan Head
- Fluoview Software
- Image Collection Panel
- Main Control Panel
- Instrument settings Tabs
- Atto Bioscience
- Atto Bioscience Confocal Microscopes
- Carv Confocal Microscopes
- Components of the Carv ContocalMicroscope
- Carv Scan Head
- Yokogawa Electric Corporation
- CSU10 Scan Head
- CSU21 Scan Head
- Perkinelmer Life Sciences
- Perkinelmer Confocal Microscopes
- Ultra View Lc1 layout
- Components of the PerkinElmer Ultra View
- Visitech International
- Main Control Panel
- Setting un the Microscope and Imaging Channels
- vSetting the Scan Control Parameters
- Adjusting Imagine Channel Settings
- Image Collection Panel
- Using the Meta Channel
- Meta Channel as a multi-Band Pass Optical Filter
- Meta Channel — Lambda Mode (Spectral Imaging)
- Extracting Image Channels from a Spectral Stack
- Emission Fingerprinting
- On-Line Emission Fingerprinting
- Leica Microsystems
- Leica Confocal Microscopes
- Components of the Leica Spectral Imaging Instrument
- Leica Tcs Sp2 Confocal Microscope
- Leica “Ics Sp2 Scan Head
- Acoustic Optical Beam Splitter (AOBS)
- Spectral Separation
- Uv Excitation
- Multi-Photon Excitation
- Transmission (DIC) Imaging
- Leica Confocal Microscope Control Software
- Main Control Panel — Monitor 1
- Changing the Desktop Knob Functions.
- “Beam Path Setting” Control Panel
- Control “Buttons” for Image Collection
- Image Collection Panel — Monitor 2
- Nikon Instruments