Insect Cell Cultures Fundamental and Applied Aspects

Bibliographic Details
Other Authors: Vlak, Just M. (Editor), de Gooijer, Cornelis D. (Editor), Tramper, Johannes (Editor), Miltenburger, Herbert G. (Editor)
Format: eBook
Language:English
Published: Dordrecht Springer Netherlands 2002, 2002
Edition:1st ed. 2002
Series:Current Applications of Cell Culture Engineering
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Insect Cell Lines
  • Development and characterization of insect cell lines
  • New approaches to insect tissue culture
  • Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene
  • Insect cell physiology
  • Insect cell cultivation: growth and kinetics
  • Medium design for insect cell culture
  • Viruses
  • Baculovirus-insect cell interactions
  • Replication patterns and cytopathology of cells infected with baculoviruses
  • Construction of baculovirus recombinants
  • Passage effect of virus infection in insect cells
  • Post-translational modifications in insect cells
  • Productivity of insect cells for recombinant proteins
  • Chaperone and foldase coexpression in the baculovirus-insect cell expression system
  • Engineering Aspects
  • Shear sensitivity of insect cells
  • Insect cell bioreactors
  • Perfusion bioreactors for the production of recombinant proteins in insect cells
  • Immobilization of insect cells
  • Modelling baculovirus infection of insect cells in culture
  • Scale up aspects of sparged insect-cell bioreactors
  • Oxygen gradients in small and big sparged insect-cell bioreactors
  • Downstream processing of insect cell cultures
  • Applications
  • Parvovirus diagnostics and vaccine production in insect cells
  • Classical swine fever virus diagnostics and vaccine production in insect cells
  • Production of multidomain complement glycoproteins in insect cells
  • Economic and Regulatory Aspects
  • Economics of baculovirus-insect cell production systems
  • Safety aspects of insect cell culture
  • Regulatory issues in the use of insect-cell culture