Optimization in Drug Discovery In Vitro Methods
Thoroughly revised and updated, Optimization in Drug Discovery: In Vitro Methods, Second Edition presents a wide spectrum of in vitro assays including formulation, plasma binding, absorption and permeability, cytochrome P450 (CYP) and UDP-glucuronosyltransferases (UGT) metabolism, CYP inhibition and...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2014, 2014
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| Edition: | 2nd ed. 2014 |
| Series: | Methods in Pharmacology and Toxicology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- In Vitro CYP Induction Using Human Hepatocytes
- Assessment of CYP3A4 Time-Dependent Inhibition in Plated and Suspended Human Hepatocytes
- Evaluation of Time-Dependent CYP3A4 Inhibition Using Human Hepatocytes
- Rapidly Distinguishing Reversible and Time-Dependent CYP450 Inhibition Using Human Liver Microsomes, Co-Incubation, and Continuous Fluorometric Kinetic Analyses
- Identification of Time-Dependent CYP Inhibitors Using Human Liver Microsomes (HLM)
- CYP Time-Dependent Inhibition (TDI) Using an IC50 Shift Assay with Stable Isotopic Labeled Substrate Probes to Facilitate Liquid Chromatography/Mass Spectrometry Analyses
- Screening for P-Glycoprotein (Pgp) Substrates and Inhibitors
- In Vitro Characterization of Intestinal Transporter, Breast Cancer Resistance Protein (BCRP)
- In Vitro Characterization of Intestinal and Hepatic Transporters: MRP2
- In Vitro Characterization of Hepatic Transporters OATP1B1 and OATP1B3
- Small Molecule Formulation Screening Strategies in Drug Discovery
- Assessment of Drug Plasma Protein Binding in Drug Discovery
- Drug Partition in Red Blood Cells
- Permeability Assessment Using 5-Day Cultured Caco-2 Cell Monolayers
- In Situ Single Pass Perfused Rat Intestinal Model
- Metabolic Stability Assessed by Liver Microsomes and Hepatocytes
- Metabolic Assessment in Alamethicin-Activated Liver Microsomes: Co-Activating CYPs and UGTs
- Phenotyping UDP-Glucuronosyltransferases (UGTs) Involved in Human Drug Metabolism: An Update
- In Vitro CYP/FMO Reaction Phenotyping
- Human Pregnane X Receptor (hPXR) Activation Assay in Stable Cell Lines
- Characterization of Constitutive Androstane Receptor (CAR) Activation
- DNA Binding (Gel Retardation Assay) Analysis for Identification of Aryl Hydrocarbon (Ah) Receptor Agonists and Antagonists
- Cell-Based Assays for Identification of Aryl Hydrocarbon Receptor (AhR) Activators
- In Vitro Characterization of Renal Transporters OAT1, OAT3, and OCT2
- General Guidelines for Setting Upan In Vitro LC/MS/MS Assay
- Metabolite Identification in Drug Discovery
- Drug, Lipid, and Acylcarnitine Profiling Using Dried Blood Spot (DBS) Technology in Drug Discovery
- In Vitro Trapping and Screening of Reactive Metabolites Using Liquid Chromatography-Mass Spectrometry
- Quantitative Assessment of Reactive Metabolites
- In Vitro Assessment of the Reactivity of Acyl Glucuronides
- In Vitro COMET Assay for Testing Genotoxicity of Chemicals
- Assessing DNA Damage Using a Reporter Gene System
- Improved AMES Test for Genotoxicity Assessment of Drugs: Preincubation Assay Using a Low Concentration of Dimethyl Sulfoxide
- Methods for Using the Mouse Lymphoma Assay to Screen for Chemical Mutagenicity and Photo-Mutagenicity