RNA Folding Methods and Protocols
Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even ato...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2014, 2014
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| Edition: | 1st ed. 2014 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Predicting RNA Structure: Advances and Limitations
- Cis-Acting 5' Hammerhead Optimization for In Vitro Transcription of Highly Structured RNAs
- RNA Structural Analysis by Enzymatic Digestion
- A Mutate-and-Map Protocol for Inferring Base Pairs in Structured RNA
- Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes
- Massively Parallel RNA Chemical Mapping with a Reduced Bias MAP-Seq Protocol
- Probing RNA Folding by Hydroxyl Radical Footprinting
- Monitoring Global Structural Changes and Specific Metal Binding Sites in RNA by In-Line Probing and Tb(III) Cleavage
- Chemical Probing of RNA in Living Cells
- A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding
- Applying UV Crosslinking to Study RNA-Protein Interactions in Multicomponent Ribonucleoprotein Complexes
- The Kinetics of Ribozyme Cleavage: A Tool to Analyze RNA Folding as a Function of Catalysis
- RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding
- Evaluation of RNA Chaperone Activity In Vivo and In Vitro Using Misfolded Group I Ribozymes
- RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis
- Detecting RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation
- RNA Folding Dynamics Using Laser Assisted Single-Molecule Refolding
- RNA Refolding Studied by Light-Coupled NMR Spectroscopy
- Exploring RNA Oligomerization and Ligand Binding by Fluorescence Correlation Spectroscopy and Small Angle X-Ray Scattering